Indirect cytotoxicity evaluations of antibacterial raw silk fabric doped with calcium, copper and zinc on fibroblasts and osteoblasts
Indirect cytotoxicity evaluations of antibacterial raw silk fabric doped with calcium, copper and zinc on fibroblasts and osteoblasts
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DOI：
10.1177/08853282211058941
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发表时间：
2022-01
影响因子：
2.9
通讯作者：
Hiroki Chigama;H. Kanetaka;Maiko Furuya;Kotone Yokota;M. Kawashita
中科院分区：
文献类型：
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作者：
Hiroki Chigama;H. Kanetaka;Maiko Furuya;Kotone Yokota;M. Kawashita
抗菌材料广泛用于预防医院获得性感染。在我们之前的报告中，金属（钙、铜或锌）掺杂的原丝织物被证明对大肠杆菌具有很强的抗菌活性。但抗菌材料有时也可能对人体产生危害；因此，在本研究中，我们间接研究了金属掺杂生丝织物提取物对成纤维细胞和成骨细胞的细胞毒性。掺钙原丝织物表现出与成纤维细胞的细胞相容性。相反，掺杂铜和锌的生丝织物显着降低了成纤维细胞的细胞密度，表明它们具有细胞毒性作用。这一观察结果可能归因于释放的铜或锌离子的高浓度。然而，掺钙、铜和锌的生丝织物并未表现出对成骨细胞的任何细胞毒性作用，因为高浓度的血清减轻了织物释放的这些金属离子的影响。因此，钙掺杂原丝织物是一种有前途的抗菌材料，且不会产生强烈的细胞毒性。这项研究将有助于设计抗菌且安全的材料。
Antibacterial materials are widely used to prevent hospital-acquired infections. In our previous report, metal (calcium, copper or zinc)-doped raw silk fabrics were shown to possess strong antibacterial activities against Escherichia coli. However, antibacterial materials may occasionally be harmful to the human body; thus, in this study, we investigated the cytotoxicities of extracts from metal-doped raw silk fabrics with respect to fibroblasts and osteoblasts indirectly. Calcium-doped raw silk fabric demonstrated cytocompatibility with fibroblasts. Contrarily, copper- and zinc-doped raw silk fabrics remarkably decreased the cell densities of fibroblasts, indicating their cytotoxic effects. This observation could be attributed to the high concentrations of the released copper or zinc ions. However, calcium-, copper- and zinc-doped raw silk fabrics did not demonstrate any cytotoxic effects on osteoblasts because a high concentration of the serum alleviated the effects of these metal ions released from the fabrics. Thus, calcium-doped raw silk fabric is a promising antibacterial material that does not induce strong cytotoxicity. This study will facilitate the design of materials that are both antibacterial and safe.