Involvement of vacuolar proton ATPase in Junin virus multiplication
Involvement of vacuolar proton ATPase in Junin virus multiplication
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DOI：
10.1007/s007050170173
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发表时间：
2001-01-01
影响因子：
2.7
通讯作者：
Coto, CE
中科院分区：
文献类型：
--
作者：
Castilla, V;Palermo, LM;Coto, CE
通过分析V-H（+）ATPAse活性特异性抑制剂对病毒增殖周期不同阶段的影响，研究了V-H（+）ATPAse在朱宁病毒（JV）复制中的作用。大环内酯类抗生素巴弗洛霉素A（1）和康卡那霉素A在感染的前两个小时内的存在导致Vero和BHK-21细胞中细胞外感染性病毒产生和病毒蛋白表达的显著减少。化合物的抑制作用主要在JV增殖周期的早期发挥，不影响病毒附着于细胞，但防止病毒穿透。观察到化合物对细胞内室酸化的抑制作用与JV产率的降低之间的相关性。在感染后不同时间加入康卡那霉素A表明，该化合物也干扰感染性颗粒释放到细胞外介质中。尽管如此，JV糖蛋白的细胞内转运到细胞膜似乎不受影响，如免疫荧光染色所示。结果证实，JV通过内吞途径进入细胞，如先前通过使用亲溶酶体化合物所建议的。
The role of vacuolar-proton ATPase (V-H(+)ATPAse) on Junin virus (JV) replication was evaluated by analyzing the effect of specific inhibitors of the enzyme activity on different steps of virus multiplication cycle. The presence of the macrolide antibiotics bafilomycin A(1) and concanamycin A during the first two hours of infection caused a significant reduction of extracellular infectious virus production and viral protein expression in Vero and BHK-21 cells. The inhibitory action of the compounds was mainly exerted at an early stage of the JV multiplication cycle, without affecting virus attachment to the cell but preventing virus penetration. A correlation between the inhibitory action of the compounds on intracellular compartments acidification and the reduction of JV yield was observed. The addition of concanamycin A at different times after infection indicated that the compound also interferes with the release of infectious particles to the extracellular medium. Although, intracellular transport of JV glycoproteins to the cell membrane, seems not to be affected as revealed by immunofluorescence staining. The results confirm that JV enters into the cell through the endocytic pathway as previously suggested by using lysosomotropic compounds.