FLIM-FRET for Cancer Applications.
FLIM-FRET for Cancer Applications.
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DOI：
10.2174/2211555203666141117221111
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Barroso M
中科院分区：
文献类型：
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作者：
Rajoria S;Zhao L;Intes X;Barroso M
光学成像测定，尤其是荧光分子测定，即使不是完全无创，也是微创的，因此是应用于活体标本的理想技术。这些荧光成像测定是生物医学领域的强大工具，因为它们可以研究生物系统中发生的各种分子和生理事件。此外，光学成像测定弥补了体外基于细胞的亚细胞过程分析与小动物模型疾病机制的体内研究之间的差距。特别是，将福斯特共振能量转移（FRET）和荧光寿命成像（FLIM）等广泛应用于显微镜的众所周知的技术应用到光学成像分析工具箱中，将对癌症进展过程中蛋白质-蛋白质相互作用的分子研究产生重大影响。这篇综述文章描述了 FLIM-FRET 在光学成像领域的应用，并讨论了它们在抗癌药物输送和癌症研究中当前和潜在的各种应用。
Optical imaging assays, especially fluorescence molecular assays, are minimally invasive if not completely noninvasive, and thus an ideal technique to be applied to live specimens. These fluorescence imaging assays are a powerful tool in biomedical sciences as they allow the study of a wide range of molecular and physiological events occurring in biological systems. Furthermore, optical imaging assays bridge the gap between the in vitro cell-based analysis of subcellular processes and in vivo study of disease mechanisms in small animal models. In particular, the application of Förster resonance energy transfer (FRET) and fluorescence lifetime imaging (FLIM), well-known techniques widely used in microscopy, to the optical imaging assay toolbox, will have a significant impact in the molecular study of protein-protein interactions during cancer progression. This review article describes the application of FLIM-FRET to the field of optical imaging and addresses their various applications, both current and potential, to anti-cancer drug delivery and cancer research.