Switchable adhesive substrates: Revealing geometry dependence in collective cell behavior
Switchable adhesive substrates: Revealing geometry dependence in collective cell behavior
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DOI：
10.1016/j.biomaterials.2011.12.012
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发表时间：
2012-03-01
期刊：
影响因子：
14
通讯作者：
Nakanishi, Jun
中科院分区：
文献类型：
--
作者：
Rolli, Claudio G.;Nakayama, Hidekazu;Nakanishi, Jun
集体细胞迁移在癌症转移和伤口愈合中发挥着重要作用，因此，已经开发了几种用于研究此类行为的体外测定法。使用光可切换表面，我们研究了最初精确控制的粘合图案的集体细胞扩张行为。非粘性聚乙二醇 (PEG) 层通过 2-硝基苯甲基基团与玻璃盖玻片缀合，这些基团在暴露于紫外线时裂解，将表面从非细胞粘附性变为细胞粘附性，而不会产生机械干扰。通过光掩模的投影曝光，产生任意形状的初始细胞附着区域。生长期后，上皮细胞片通过第二次照明从其限制中释放出来，从而允许集体细胞扩张。我们对上皮细胞的实验表明，簇大小和边界曲率调节细胞片的扩张和前导细胞的形成。在一定的团簇尺寸下，膨胀行为的特征发生变化，核心细胞几乎不受边界释放的影响。通过甜甜圈状的环状结构，我们证明了沿外凸边界和沿内凹边界的细胞行为之间的对称性断裂。此外，我们观察到集体迁移特征受到细胞片的初始孵育时间的调节。 (C) 2011 Elsevier Ltd. 保留所有权利。
Collective cell migration plays a major role in cancer metastasis and wound healing, therefore, several in vitro assays for studying such behavior have been developed. Using photoswitchable surfaces, we studied collective cell expansion behavior from initially precisely controlled adhesive patterns. A non-adhesive poly(ethylene glycol) (PEG) layer is conjugated to a glass coverslip via 2-nitrobenzyl groups, which cleave upon exposure to UV light, changing the surface from non-cell-adhesive to cell-adhesive without mechanical interference. Initial cell attaching areas are generated in arbitrary shapes via projection exposure through a photomask. After a growth phase, epithelial cell sheets are released from their confinement by a second illumination allowing for collective cell expansion. Our experiments with epithelial cells show that cluster size and boundary curvature modulate the expansion of the cell sheet and the formation of leader cells. At a certain cluster size, characteristics of the expansion behavior change and cells in the core are hardly affected by the boundary release. With donut-like ring structures, we demonstrate a break in symmetry between the behavior of cells along the outer convex boundary and along the inner concave boundary. Additionally, we observe that collective migration characteristics are modulated by the initial incubation time of the cell sheet. (C) 2011 Elsevier Ltd. All rights reserved.