Chip calorimetry and biomagnetic separation: Fast detection of bacterial contamination at low cell titers
Chip calorimetry and biomagnetic separation: Fast detection of bacterial contamination at low cell titers
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DOI：
10.1002/elsc.201200029
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发表时间：
2012-11
影响因子：
2.7
通讯作者：
J. Lerchner;Anne Schulz;T. Poeschel;A. Wolf;Tom Hartmann;F. Mertens;E. Boschke
中科院分区：
文献类型：
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作者：
J. Lerchner;Anne Schulz;T. Poeschel;A. Wolf;Tom Hartmann;F. Mertens;E. Boschke
我们提出了一种新的芯片量热计，用于快速定量测量附着在磁珠上的微生物的代谢热率。在生物磁分离（BMS）实验中，固定在非特异性功能化微球上的大肠杆菌K12在37℃下的比热率约为1 pW /细胞。因此，至少需要2 × 104个细菌才能超过20 nW的量热信号分辨率。如果待分析样品的原始体积为4ml，则应检测到小于104个细胞mL - 1的细菌。在实践中，我们实现了大约2 × 104个细胞mL−1的检测。本文提出的方法在生物膜的研究和生物分子相互作用的研究中也有一定的应用前景。
We present a new chip calorimeter for fast and quantitative measurement of metabolic heat rates of microorganisms attached to magnetic beads. In biomagnetic separation (BMS) experiments, Escherichia coli K12 immobilized on nonspecifically functionalized beads has a specific heat rate of around 1 pW per cell at 37°C. Therefore, at least 2 × 104 bacteria are required to exceed the calorimetric signal resolution of 20 nW. If the samples to be analyzed have the original volume of 4 mL, bacteria at less than 104 cells mL−1 should be detectable. In practice, we achieved the detection of approximately 2 × 104 cells mL−1. The method presented here might also find some applications in the investigation of biofilms and study of biomolecular interactions.