Involvement of ryanodine receptors in pacemaker Ca2+ oscillation in murine gastric ICC.
Involvement of ryanodine receptors in pacemaker Ca2+ oscillation in murine gastric ICC.
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DOI：
10.1016/j.bbrc.2005.01.029
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发表时间：
2005-03
影响因子：
3.1
通讯作者：
Hong-nian Liu;S. Ohya;Jing Wang;Y. Imaizumi;S. Nakayama
中科院分区：
文献类型：
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作者：
Hong-nian Liu;S. Ohya;Jing Wang;Y. Imaizumi;S. Nakayama
使用从小鼠的胃平滑肌组织的细胞簇制备，我们测量了细胞内Ca 2+振荡Cajal间质细胞（ICCs）在硝苯地平的存在下。ICCs的起搏[Ca ~（2+）] i活性被咖啡因应用显著抑制，并在洗脱后恢复。应用Ryanodine或FK-506均不可逆地终止了起搏器[Ca ~（2+）] i活性。平滑肌组织的免疫染色显示，c-Kit免疫阳性细胞（在肌间神经丛中形成网状结构细胞，相当于ICC）明确表达兰尼碱受体（RyR）。RT-PCR结果显示ICCs主要表达3型RyR（RyR 3）。此外，检测到FK结合蛋白12和12.6，这两个蛋白都将与RyR 3相互作用。总之，我们提供了第一个证据的RyR产生起搏器活动在胃动力的重要贡献。类似的机制可以解释分布在自主神经系统中的平滑肌组织中观察到的自发节律性。
Using a cell cluster preparation from the stomach smooth muscle tissue of mice, we measured intracellular Ca2+oscillations in interstitial cells of Cajal (ICCs) in the presence of nifedipine. Pacemaker [Ca2+]iactivity in ICCs was significantly suppressed by caffeine application and restored after washout. Application of either ryanodine or FK-506 terminated the pacemaker [Ca2+]iactivity irreversibly. Immunostaining of smooth muscle tissue showed that c-Kit-immunopositive cells (that form network-like structure cells in the myenteric plexus, equivalent to ICCs) clearly express ryanodine receptors (RyR). RT-PCR revealed that ICCs (identified with c-Kit-immunoreactivity) predominantly express type 3 RyR (RyR3). Furthermore, the FK-binding proteins 12 and 12.6, both of which would interact with RyR3, were detected. In conclusion, we provide first evidence for the essential contribution of RyR to generating pacemaker activity in gastric motility. Similar mechanisms might account for spontaneous rhythmicity seen in smooth muscle tissues distributed in the autonomic nervous system.