The microbial community in the feces of the white rhinoceros (Ceratotherium simum) as determined by barcoded pyrosequencing analysis.
The microbial community in the feces of the white rhinoceros (Ceratotherium simum) as determined by barcoded pyrosequencing analysis.
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DOI：
10.1371/journal.pone.0070103
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Zhu W
中科院分区：
文献类型：
--
作者：
Bian G;Ma L;Su Y;Zhu W
作为一种非反刍的食草动物，白色犀牛具有通过后肠中的微生物发酵来利用纤维植物物质的能力。到目前为止，还没有使用分子技术研究白色犀牛肠道微生物群的报道。我们使用条形码焦磷酸测序来表征从5头白色犀牛的粪便样本中获得的105，651个16S rRNA基因序列。结果表明，厚壁菌门和拟杆菌门是样品中的优势门，其中大部分是未分类的细菌。用药物治疗的一只动物的微生物群与其他健康动物的微生物群不同，并且以气球菌相关细菌为主。健康犀牛体内的核心微生物群以厚壁菌门和拟杆菌门为主，以瘤胃球菌科、毛螺菌科、Rikenellaceae和Prevotellaceae科为代表。目前的工作提供了一个系统发育的框架，了解复杂的微生物群落的犀牛，但是，需要进一步的研究，以连接独特的微生物群与它们的消化作用在后肠的白色犀牛。
As a non-ruminant herbivore, the white rhinoceros has the ability to utilize fibrous plant matter through microbial fermentation in the hindgut. So far, there has been no report using molecular techniques to study the gut microbiota of the white rhinoceros. We used barcoded pyrosequencing to characterize 105,651 sequences of 16S rRNA genes obtained from fecal samples from five white rhinoceroses. Results showed that Firmicutes and Bacteroidetes were the predominant phyla in the samples, which were comprised largely of unclassified bacteria. The microbiota of one animal treated with drug therapy differed from those in other healthy animals, and was dominated by Aerococcus -related bacteria. The core microbiota in the healthy rhinoceros were dominated by phyla Firmicutes and Bacteroidetes, represented by the Ruminococcaceae, Lachnospiraceae, Rikenellaceae and Prevotellaceae families. The present work provides a phylogenetic framework for understanding the complex microbial community of the rhinoceros; however, further studies are required to link the distinctive microbiota with their digestive role in the hindgut of the white rhinoceros.
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DOI：
10.1038/ismej.2012.50
发表时间：
2012-11
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
3.7
作者：
Jami E;Mizrahi I
通讯作者：
Mizrahi I
影响因子：
2.7
作者：
HENRY, JS;LANCE, VA;CONLON, JM
通讯作者：
CONLON, JM
影响因子：
2.7
作者：
GOOD, IJ
通讯作者：
GOOD, IJ
DOI：
10.1016/j.jmii.2011.09.010
发表时间：
2012-04-01
影响因子：
7.4
作者：
Chen, Liang-Yu;Yu, Wen-Chung;Liu, Cheng-Yi
通讯作者：
Liu, Cheng-Yi