Postnatal development of rat dentate gyrus: effects of methylazoxymethanol administration
Postnatal development of rat dentate gyrus: effects of methylazoxymethanol administration
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DOI：
10.1016/s0047-6374(01)00359-1
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发表时间：
2002-03-15
影响因子：
5.3
通讯作者：
Del Grande, P
中科院分区：
文献类型：
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作者：
Ciaroni, S;Cecchini, T;Del Grande, P
为了探讨生后神经发生在大鼠齿状回颗粒细胞数量控制中的作用，我们在P3、P5、P7、P9上给予甲氧基甲醇(NiAM)，这是一种能阻止细胞分裂的药物，此时颗粒细胞最多。在P16和P90检测MAM对增殖前体细胞和颗粒细胞数量的影响。用5-溴-2‘-脱氧尿嘧啶核苷标记增殖细胞，用神经元标记物TUC-4、PSA-NCAM、Calbindin D28K和胶质标记物GFAP免疫组织化学标记细胞表型。在16天龄时，我们观察到MAM处理的大鼠BrdU阳性细胞显着减少。与对照组相比，颗粒细胞的密度和数量一直在下降。90d时，治疗组大鼠齿状回完全恢复，神经元密度、神经元总数、BrdU和TUC4阳性细胞数与对照组无明显差异。MAM治疗的大鼠在水迷宫中的表现没有明显的缺陷。这些数据表明，在给予新生儿MAM后，齿状回能够重建增殖区和重建颗粒细胞层。(C)2002爱思唯尔科学爱尔兰有限公司。保留所有权利。
In order to investigate the role of postnatal neurogenesis in granule cell number control in the rat dentate gyrus, we administered Methylazoxymethanol (NIAM), a drug able to prevent cells from dividing, on P3, P5, P7, P9, when the most granule cells are produced. The effect of MAM on the number of proliferating precursors and of granule cells was examined at P16 and P90. We used 5-bromo-2'-deoxyuridine administration to label proliferating cells and immunohistochemistry to characterize the cell phenotype using neuron markers TUC 4, PSA-NCAM, Calbindin D28K and glial marker GFAP. At 16 days of age in MAM-treated rats we observed a significant decrease of BrdU-positive cells. Consistently, a decrease in density and number of granule cells was found compared to the controls. At 90 days the dentate gyrus of treated rats showed a complete recovery: no differences in the density, total number of neurons, the BrdU- and TUC 4-positive cells were revealed with respect to the controls. No deficits were evident in performance on the water maze in MAM-treated rats. These data suggest that the dentate gyrus is able to re-establish the proliferative zone and to rebuild the granule cell layer following neonatal MAM administration. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.