Bilayered near-infrared fluorescent nanoparticles based on low molecular weight PEI for tumor-targeted in vivo imaging
Bilayered near-infrared fluorescent nanoparticles based on low molecular weight PEI for tumor-targeted in vivo imaging
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基于低分子量PEI的双层近红外荧光纳米粒子用于肿瘤靶向体内成像
DOI：
10.1007/s11051-014-2784-5
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发表时间：
2014-12-01
影响因子：
2.5
通讯作者：
Wu, Daocheng
中科院分区：
文献类型：
--
作者：
Liu, Hao;Li, Ke;Wu, Daocheng
为了提高肿瘤在体内的荧光成像效果，采用低分子量硬脂酸接枝聚乙烯亚胺和透明质酸（DiR- pgsha纳米粒子）制备了包裹亲脂性近红外（NIR）荧光染料1,1′-二十八烷基-3,3,3′，3′-四甲基lindotri-carbocyanine ioide （DiR）的两层纳米粒子，并将其作为一种新型的近红外荧光纳米探针用于体内肿瘤靶向光学成像。通过透射电子显微镜（TEM）、红外光谱（IR）、紫外可见吸收光谱和荧光发射光谱对纳米颗粒进行了表征。分别采用MTT法和组织学法检测其体外细胞毒性和体内肝毒性。使用Carestream成像系统对DiR-PgSHA纳米颗粒进行体内近红外荧光成像。根据TEM图像，DiR-PgSHA纳米颗粒为球形，直径约为50 nm。根据MTT测定，DiR-PgSHA纳米颗粒在体外具有低细胞毒性，在体内具有低肝毒性，经组织学研究确定。在pH值为5 ~ 9的溶液中，DiR-PgSHA纳米粒子的荧光发射是稳定的，在碱性pH范围内，发射最大值只有轻微的蓝移。在荷瘤小鼠的光学成像中，DiR-PgSHA纳米颗粒显示出明显的肿瘤靶向能力。这些结果表明，DiR-PgSHA纳米颗粒是一种极好的生物相容性纳米探针，用于体内肿瘤靶向近红外荧光成像，具有潜在的临床应用价值。
To improve the tumor fluorescent imaging results in vivo, bilayered nanoparticles encapsulating a lipophilic near-infrared (NIR) fluorescent dye 1,1'-dioctadecyl-3,3,3',3'-tetramethylindotri-carbocyanine iodide (DiR) were prepared using low molecular weight stearic acid-grafted polyethyleneimine and hyaluronic acid (DiR-PgSHA nanoparticles), which were investigated as a novel NIR fluorescent nanoprobe for in vivo tumor-targeted optical imaging. These nanoparticles were characterized by transmission electron microscopy (TEM), infrared (IR) spectra, UV-visual absorption, and fluorescent emission spectra. Their cytotoxicity in vitro and hepatotoxicity in vivo were tested by MTT assay and histological study, respectively. In vivo NIR fluorescence imaging of the DiR-PgSHA nanoparticles was performed using a Carestream imaging system. The DiR-PgSHA nanoparticles were sphere shaped with a diameter of approximately 50 nm according to the TEM images. The DiR-PgSHA nanoparticles had a low cytotoxicity in vitro according to the MTT assay and low hepatotoxicity in vivo as determined in histological studies. The fluorescent emission of DiR-PgSHA nanoparticles was stable in pH values of 5-9 in solution, with only slight blue-shifts of the emission maxima at the basic pH range. The DiR-PgSHA nanoparticles exhibited a substantial tumor-targeting ability in the optical imaging with the use of tumor-bearing mice. These results demonstrated that the DiR-PgSHA nanoparticle is an excellent biocompatible nano-probe for in vivo tumor-targeted NIR fluorescence imaging with a potential for clinical applications.