Human SFMBT is a transcriptional repressor protein that selectively binds the N-terminal tail of histone H3
Human SFMBT is a transcriptional repressor protein that selectively binds the N-terminal tail of histone H3
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DOI：
10.1016/j.febslet.2007.06.025
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发表时间：
2007-07-10
期刊：
影响因子：
3.5
通讯作者：
Rice, Judd C.
中科院分区：
文献类型：
--
作者：
Wu, Shumin;Trievel, Raymond C.;Rice, Judd C.
人SFMBT(HSFMBT)被认为是一种多梳(PcG)蛋白。与其他PcG蛋白相似，我们发现hSFMBT具有很强的转录抑制活性。此外，hSFMBT通过直接和选择性地结合组蛋白H3的N端尾巴，定位于细胞核，在那里它与染色质强烈结合。重要的是，我们发现hSFMBT的四个串联MBT重复足以进行核基质结合、N末端尾部H3结合，并需要转录抑制。这些发现表明，串联的MBT重复形成了hSFMBT生物活性所需的功能结构，并预测了其他含有MBT结构域的蛋白质的类似性质。(C)2007年欧洲生化学会联合会。爱思唯尔出版，版权所有。
Human SFMBT (hSFMBT) is postulated to be a Polycomb (PcG) protein. Similar to other PcG proteins, we found that hSFMBT displays robust transcriptional repressor activity. In addition, hSFMBT localized to the nucleus where it strongly associates with chromatin by directly and selectively binding the N-terminal tail of histone H3. Importantly, we discovered that the four tandem MBT repeats of hSFMBT were sufficient for nuclear matrix-association, N-terminal tail H3 binding, and required for transcriptional repression. These findings indicate that the tandem MBT repeats form a functional structure required for biological activity of hSFMBT and predict similar properties for other MBT domain-containing proteins. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.