Transformation of dissolved organic matter by oxidative polymerization with horseradish peroxidase
Transformation of dissolved organic matter by oxidative polymerization with horseradish peroxidase
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DOI：
10.2166/wst.2010.248
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发表时间：
2010-01-01
影响因子：
2.7
通讯作者：
Ko, S. O.
中科院分区：
文献类型：
--
作者：
Jee, S. H.;Kim, Y. J.;Ko, S. O.
溶解性有机物（DOM）对污染物的迁移和归宿具有重要的多相影响，是给水消毒副产物的前体物质，具有潜在的危害性。DOM特性的变化，特别是氧化聚合，可能会导致不同的行为与许多污染物的相互作用。本工作的目的是验证过氧化物酶对腐殖酸和富里酸物质的氧化聚合的催化作用，通过检查的结构特征。研究了辣根过氧化物酶和过氧化氢催化氧化聚合法转化腐殖酸（HA）和富里酸（FA）。采用体积排阻色谱、激发发射矩阵光谱、同步荧光光谱和红外光谱等方法对HA和FA的结构转变进行了评价。HA和FA的相对分子质量连续变化，在8h后达到最大值。HA和FA的相对分子质量随辣根过氧化物酶和过氧化氢用量的增加而成比例增加，表明HA和FA转化为更大更复杂的分子。光谱分析结果表明，HA和FA结构中含有较强的多环芳香结构，芳香环数较多，共轭度较高。
Dissolved organic matter (DOM) has significant influence on the transport and fate of contaminants in multiple phases and it has potential hazard by acting as a precursor of disinfection by-products in water supply. The changes in DOM characteristics, especially by oxidative polymerization might result in different behaviour in the interaction with many contaminants. The aim of this work was to verify the catalytic effects of peroxidase on oxidative polymerization of humic and fulvic substances by examination of the structural characteristics. Transformation of humic acid (HA) and fulvic acid (FA) by oxidative polymerization catalyzed by horseradish peroxidase and hydrogen peroxide were investigated. Size exclusion chromatography, excitation emission matrices spectra (EEMs), synchronous fluorescence spectra, and infrared spectroscopy was used to evaluate the structural transformation of HA and FA. Molecular weight of HA and FA was continuously changed and their weight-average molecular weight (MWw) reached maximum after 8 h. The MWw of HA and FA were proportionally increased with a dosage of horseradish peroxidase and hydrogen peroxide, indicating that HA and FA was transformed into larger and complex molecules. Spectroscopic results indicated that HA and FA structure contains strong polycyclic aromatic structures with more aromatic rings and a higher degree of conjugation.