Low-dose GH supplementation reduces the TLR2 and TNF-α expressions in visceral fat
Low-dose GH supplementation reduces the TLR2 and TNF-α expressions in visceral fat
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DOI：
10.1016/j.bbrc.2008.01.035
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发表时间：
2008-03-28
影响因子：
3.1
通讯作者：
Saito, Yasushi
中科院分区：
文献类型：
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作者：
Kubota, Yoshitaka;Unoki, Hiroyuki;Saito, Yasushi
TLR 2/TNF-α共表达脂肪细胞的数量增加与胰岛素抵抗的发生有关。我们在此显示了低剂量生长激素（GH）补充对于使用不同种类的高脂饮食喂养的小鼠模型调节内脏脂肪中TLR 2和TNF-α表达的意义。低剂量GH补充减少了高脂喂养小鼠中TLR 2/TNF-α共表达脂肪细胞的数量增加。IGF-1的中和作用消除了GH过度表达小鼠补充GH对TLR 2表达的影响。IGF-1，而不是GH，抑制FFA诱导的TLR 2和TNF-α在3 T3-L1细胞中的表达。最后，低剂量GH补充降低了TLR 2的表达，而对ob/ob小鼠的内脏脂肪体积没有明显的变化。这些结果表明，低剂量GH补充可能通过IGF-1的作用抑制高脂诱导的脂肪细胞向TLR 2/TNF-α共表达细胞的变化。(C)2008年爱思唯尔公司All rights reserved.
The increased population of TLR2/TNF-alpha co-expressing adipocytes is associated with the development of insulin resistance. We have herein shown the significance of low-dose growth hormone (GH) supplementation for the regulation of TLR2 and TNF-alpha expressions in visceral fat using different kinds of mouse models fed with a high-fat diet. Low-dose GH supplementation reduced the increased population of TLR2/TNF-alpha co-expressing adipocytes in high-fat fed mice. The neutralization of IGF-1 abolished the effect of GH supplementation on the TLR2 expression using GH-overexpressing mice. IGF-1, but not GH, inhibited the FFA-induced TLR2 and TNF-alpha expression in 3T3-L1 cells. Finally, low-dose GH supplementation reduced the TLR2 expression without an obvious change in the visceral fat volume in ob/ob mice. These results indicate that low-dose GH supplementation possibly inhibits the high-fat induced change of the adipocytes to TLR2/TNF-alpha co-expressing cells through the action of 1GF-1. (C) 2008 Elsevier Inc. All rights reserved.