A Near-Infrared Neutral pH Fluorescent Probe for Monitoring Minor pH Changes: Imaging in Living HepG2 and HL-7702 Cells
A Near-Infrared Neutral pH Fluorescent Probe for Monitoring Minor pH Changes: Imaging in Living HepG2 and HL-7702 Cells
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用于监测微小 pH 变化的近红外中性 pH 荧光探针：HepG2 和 HL-7702 活细胞中的成像
DOI：
10.1021/ja809149g
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发表时间：
2009-03-04
影响因子：
15
通讯作者：
Wang, Xu
中科院分区：
文献类型：
--
作者：
Tang, Bo;Yu, Fabiao;Wang, Xu
开发了一种近中性pH近红外(NIR)荧光探针，它利用荧光团-间隔基-受体分子框架，通过快速的光诱导电子转移过程来调节荧光发射强度。我们的策略是选择高消光系数的近红外荧光染料三碳菁(Cy)作为荧光团，4‘-(氨基甲基苯基)-2，2’：6‘，2’‘-三吡啶(TPY)作为受体。PH滴定结果表明，TPY-Cy可以监测微小的生理pH波动，其pK(A)接近生理pH的7.10，对细胞内pH的研究具有一定的参考价值。该探针对6.70-7.90范围内的微小pH波动做出快速线性响应，并对pH变化表现出很强的依赖性。如预期的那样，该探针成功地实现了细胞pH的实时成像和活体HepG2和HL-7702细胞的pH检测。结果表明，该探针有效地避免了生物体系中自然荧光和天然细胞物种的影响，同时具有高灵敏度、良好的光稳定性和良好的细胞膜通透性。
A near-neutral pH near-infrared (NIR) fluorescent probe utilizing a fluorophore-spacer-receptor molecular framework that can modulate the fluorescence emission intensity through a fast photoinduced electron-transfer process was developed. Our strategy was to choose tricarbocyanine (Cy), a NIR fluorescent dye with high extinction coefficients, as a fluorophore, and 4'-(aminomethylphenyl)-2,2':6',2 ''-terpyridine (Tpy) as a receptor. The pH titration indicated that Tpy-Cy can monitor the minor physiological pH fluctuations with a pK(a) of similar to 7.10 near physiological pH, which is valuable for intracellular pH researches. The probe responds linearly and rapidly to minor pH fluctuations within the range of 6.70-7.90 and exhibits strong dependence on pH changes. As expected, the real-time imaging of cellular pH and the detection of pH in situ was achieved successfully in living HepG2 and HL-7702 cells by this probe. It is shown that the probe effectively avoids the influence of autofluorescence and native cellular species in biological systems and meanwhile exhibits high sensitivity, good photostability, and excellent cell membrane permeability.