Establishment of a Streptococcus pneumoniae nasopharyngeal colonization model in adult mice.
Establishment of a Streptococcus pneumoniae nasopharyngeal colonization model in adult mice.
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DOI：
10.1006/mpat.1997.0142
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发表时间：
1997-09
影响因子：
3.8
通讯作者：
H. Y. Wu;A. Virolainen;B. Mathews;J. King;M. Russell;D. Briles
中科院分区：
文献类型：
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作者：
H. Y. Wu;A. Virolainen;B. Mathews;J. King;M. Russell;D. Briles
人鼻咽携带肺炎链球菌是肺炎球菌的主要天然宿主，被认为是几乎所有肺炎球菌疾病的前奏。如果能够大大减少携带，肺炎球菌的传播和疾病就可以在很大程度上消除。为了促进对携带的重要机制的研究，并确定免疫原可以引起对携带的保护，我们描述了一个成年小鼠鼻咽携带模型。未麻醉的小鼠鼻内接种10微升液体的肺炎球菌。鼻咽携带荚膜型3、4、6A、6B、14、19和23株。随时间的推移，携带的肺炎球菌数量相对独立于接种剂量；结果表明，从接种后1天到2周，肺炎球菌的恢复取决于定植，而不仅仅是暂时的污染。为了确保最大比例的小鼠携带，而不引起败血症或死亡，应接种10(7)个菌落形成单位（cfu）。在这个模型中，通常没有观察到携带菌血症或败血症，甚至在静脉注射时也观察到携带菌，即使菌株是无毒的。该模型应该有助于识别引起保护的抗原，因为用热杀肺炎球菌或肺炎球菌裂解物进行鼻内免疫可以防止携带菌。
Human nasopharyngeal carriage of Streptococcus pneumoniae constitutes the major natural reservoir of pneumococci and is thought to be the prelude to virtually all pneumococcal disease. If carriage could be greatly reduced, pneumococcal transmission and disease could be largely eliminated. To facilitate the studies of mechanisms important in carriage and to identify immunogens that can elicit protection against carriage, we characterized an adult mouse model of nasopharyngeal carriage. Non-anaesthetized mice were inoculated intranasally with pneumococci in 10 microl of fluid. Nasopharyngeal carriage was observed with strains of capsular types 3, 4, 6A, 6B, 14, 19, and 23. Carriage was stable over time, and the numbers of pneumococci carried were relatively independent of inoculation dose; findings which indicate that the recovery of pneumococci from 1 day to 2 weeks post inoculation was dependent on colonization, rather than just temporary contamination. To ensure carriage in the largest percentage of mice, without causing sepsis or death, inoculations of 10(7) colony forming units (cfu) should be used. In this model, carriage was generally observed without concomitant bacteremia or sepsis and carriage was observed even with strains that were avirulent when injected i.v. The model should be useful for the identification of protection-eliciting antigens, since intranasal immunization with heat-killed pneumococci or lysates of pneumococci protected against carriage.