Anti-Oxidant, Anti-Inflammatory and Antinociceptive Properties of the Acetone Leaf Extract of Vernonia Amygdalina in Some Laboratory Animals
Anti-Oxidant, Anti-Inflammatory and Antinociceptive Properties of the Acetone Leaf Extract of Vernonia Amygdalina in Some Laboratory Animals
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DOI：
10.5681/apb.2014.087
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发表时间：
2014-12-01
影响因子：
3.6
通讯作者：
Oyagbemi, Ademola Adetokunbo
中科院分区：
文献类型：
--
作者：
Adedapo, Adeolu Alex;Aremu, Olujoke Janet;Oyagbemi, Ademola Adetokunbo
用途：杏仁斑斑鸠菊是一种重要的药用植物，其鲜叶富含维生素和盐，因此在人类饮食中很有价值。本研究评价其丙酮叶提取物的抗氧化、抗炎和镇痛活性，以验证其民间传说用途。方法：将磨碎的植物材料（200g）溶解在1L丙酮中48h，过滤，然后用旋转蒸发仪干燥，制备丙酮提取物，然后用于药理研究。使用标准植物化学方法来测试植物中植物活性化合物的存在。在小鼠中进行急性毒性以确定安全使用剂量。使用角叉菜胶和组胺诱导大鼠水肿进行抗炎活性，并使用乙酸诱导的扭体试验和福尔马林诱导的舔爪试验进行镇痛活性。使用 ABTS、DPPH、FRAP 和总多酚在体外评估抗氧化活性。 结果：本研究结果表明，100 和 200 mg/kg 剂量的丙酮提取物可显着减少角叉菜胶和组胺引起的水肿。在镇痛试验中也观察到了类似的效果，与研究中使用的参考药物吲哚美辛的效果相当。结论：抗氧化效果也很好，药理活性可能是由于植物中含有多酚和其他植物化学物质。因此，这项研究可能验证了这种植物作为药物和营养剂的民间用途。
Purpose: Vernonia amygdalina is a medicinal plant of great importance that has its fresh leaves rich in vitamins and salt hence, it is valuable in human diet. The anti-oxidant, anti-inflammatory and analgesic activities of its acetone leaf extract were evaluated in this study to validate its folkloric use.Methods: The acetone extract is prepared by dissolving ground plant materials (200g) in 1 L of acetone for 48 h, filtered, and then dried using rotary evaporator before it is used for the pharmacological investigations. Standard phytochemical methods were used to test for the presence of phytoactive compounds in the plant. Acute toxicity was carried out in mice to determine safe doses for use. The anti-inflammatory activities were conducted using carrageenan and histamine to induce oedema in rats while analgesic activities were embarked upon using acetic acid-induced writhing test and formalin-induced paw lick test. The antioxidant activities were assessed in vitro using ABTS, DPPH, FRAP and total polyphenolics.Results: The results from this study showed that the 100 and 200 mg/kg doses of the acetone extract caused significant reduction in oedema induced by both carrageenan and histamine. Similar effect was observed in analgesic tests which were comparable to that of indomethacin, the reference drug used in the study.Conclusion: The anti-oxidant effects were also good and the pharmacological activities may be due to the presence of polyphenols and other phytochemicals contained in the plant. The study may have thus validated the folkloric use of this plant as a medicinal and nutritional agent.