EXPRESSION, SELF-ASSEMBLY, AND ANTIGENICITY OF A SNOW MOUNTAIN AGENT-LIKE CALICIVIRUS CAPSID PROTEIN
EXPRESSION, SELF-ASSEMBLY, AND ANTIGENICITY OF A SNOW MOUNTAIN AGENT-LIKE CALICIVIRUS CAPSID PROTEIN
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DOI：
10.1128/jcm.33.6.1452-1455.1995
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发表时间：
1995-06-01
影响因子：
9.4
通讯作者：
PICKERING, LK
中科院分区：
文献类型：
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作者：
JIANG, X;MATSON, DO;PICKERING, LK
用含有人类杯状病毒墨西哥株MX病毒衣壳基因的重组杆状病毒感染昆虫细胞，在电子显微镜下观察到这些重组MX(RMX)颗粒与重组诺瓦克病毒(RNV)颗粒形态相似，含有单一的衣壳蛋白，分子量为57,000，略小于RNV的衣壳蛋白。该蛋白由感染雪山病毒的志愿者血清免疫沉淀，但与感染NV的志愿者血清反应弱，该蛋白在RNV抗原免疫试验(EIA)中不与RNV免疫的动物的高免抗血清反应。在用RMX微粒作为抗原进行EIA时，检测到感染雪山病毒和夏威夷病毒的志愿者的血清反应，该EIA还在分离MX病毒的儿童血清中检测到免疫反应，在墨西哥队列儿童的血清中发现MX病毒抗体的高流行率。
Virus-like particles were produced in insect cells infected with a recombinant baculovirus containing the capsid gene of MX virus, a Mexican strain of human calicivirus, These recombinant MX (rMX) particles were morphologically similar to recombinant Norwalk virus (rNV) particles as observed under an electron microscope and contained a single capsid protein with a molecular weight of 57,000, which was slightly smaller than that of rNV, This protein was immunoprecipitated by sera from volunteers infected with the Snow Mountain agent, but it reacted weakly with sera from volunteers infected with NV, This protein did not react with hyperimmune antisera from animals immunized with rNV in the rNV antigen enzyme immunoassay (EIA). Seroresponses were detected from volunteers infected with Snow Mountain agent and Hawaii agent when the rMX particles were used as antigen in an EIA, This EIA also detected an immune response in the sera of child from whom the MX virus was isolated, and a high prevalence of antibody to MX virus was found in the sera of a cohort of Mexican children.