Isolation and Characterization of an Iron-Containing Superoxide Dismutase From Water Lily, Nuphar luteum.
Isolation and Characterization of an Iron-Containing Superoxide Dismutase From Water Lily, Nuphar luteum.
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DOI：
10.1104/pp.69.1.161
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发表时间：
1982
期刊：
影响因子：
7.4
通讯作者：
M. Salin;S. Bridges
中科院分区：
文献类型：
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作者：
M. Salin;S. Bridges
从睡莲Nuphar luteum(L.)叶片中纯化得到一种氰化物不敏感的超氧化物歧化酶(EC 1.15.1.1)。锡伯斯。和Smith亚种。大叶(小)豆。该酶的相对分子质量为46,000，由两个大小相等的亚基组成。金属分析显示，该蛋白质每个二聚体含有约1克铁原子。含铁超氧化物歧化酶对H(2)O(2)和叠氮同样敏感。该蛋白的抗体与从真核生物油菜中分离的铁超氧化物歧化酶或藻类提取物不发生交叉反应。
A cyanide-insensitive superoxide dismutase (EC 1.15.1.1) was purified from leaves of the water lily Nuphar luteum (L.) Sibth. and Smith Subsp. macrophyllum (Small) Beal. The enzyme had a molecular weight of 46,000 and was composed of two equally sized subunits. Metal analysis showed the protein to contain about 1 gram atom of iron per dimer. The iron-containing superoxide dismutase was sensitive to H(2)O(2) as well as to azide. Antibody to the protein did not cross-react with iron superoxide dismutase isolated from the eucaryote Brassica or with algal extracts.