Suppression of postprandial blood glucose elevation by buckwheat (Fagpopyrum esculentum) albumin hydrolysate and identification of the peptide responsible to the function
Suppression of postprandial blood glucose elevation by buckwheat (Fagpopyrum esculentum) albumin hydrolysate and identification of the peptide responsible to the function
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DOI：
10.1016/j.fshw.2022.03.026
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发表时间：
2022-07-01
影响因子：
7
通讯作者：
Kumagai, Hitomi
中科院分区：
文献类型：
--
作者：
Ninomiya, Kazumi;Yamaguchi, Yusuke;Kumagai, Hitomi
本研究的目的是评估荞麦白蛋白水解物对餐后高血糖的抑制作用，并确定负责该功能的肽。采用消化酶法制备荞麦蛋白水解物，并与可溶性淀粉一起口服给药。在口服给药后90分钟内从尾静脉采集血液，以测量血糖和血浆胰岛素水平。肽与？？通过凝胶过滤层析从荞麦水解物中纯化淀粉酶抑制活性，淀粉酶亲和色谱法和高效液相色谱法（HPLC）。用蛋白质测序仪对该肽的氨基酸序列进行了鉴定，并与荞麦基因组数据库中的氨基酸序列进行了比较。荞麦白蛋白水解物显着抑制血糖水平的升高淀粉给药后15分钟。肽的氨基酸序列与？？-在分子量为17.8 kDa和理论pI为4.77的亲本蛋白中，淀粉酶抑制活性为YVEPDCGNLGCCYHC。荞麦清蛋白中亲本蛋白的氨基酸序列、分子量和pI均与荞麦清蛋白中的亲本蛋白不同。淀粉酶抑制剂。这项研究表明，小说？？荞麦白蛋白中淀粉酶抑制剂是抑制餐后血糖升高功能性食品材料的潜在候选者。?? 2022北京市食品科学研究院。Elsevier B. V.代表KeAi Communications Co.提供出版服务，这是一个在CC BY-NC-ND许可证下的开放获取文章（http：//creativecommons.org/licenses/by-nc-nd/4.0/）。
The objective of this study is to evaluate the suppressive effect of buckwheat-albumin hydrolysate on postprandial hyperglycemia and identify the peptide responsible to the function. Buckwheat-albumin hydrolysate was prepared by using digestive enzymes and was orally administered to rats together with soluble starch. The blood was taken from the tail vein up to 90 min after oral administration to measure blood-glucose and plasma-insulin levels. The peptide with ??-amylase inhibitory activity was purified from the buckwheathydrolysate by gel-filtration chromatography, ??-amylase affinity chromatography, and high performance liquid chromatography (HPLC). The amino-acid sequence of the peptide was identified by a protein sequencer and was compared with that in the buckwheat-genome database. Buckwheat-albumin hydrolysate significantly suppressed the elevation of blood glucose level 15 min after starch administration. The amino-acid sequence of the peptide with ??-amylase inhibitory activity was YVEPDCGNLGCCYHC in the parental protein of molecular mass 17.8 kDa and theoretical pI 4.77. The amino-acid sequence, molecular weight, and pI of the parental protein in buckwheat albumin were different from those of ??-amylase inhibitor in wheat albumin. This study suggests that the novel ??-amylase inhibitor identified in buckwheat albumin is a potential candidate for a functional food material to suppress postprandial blood glucose elevation. ?? 2022 Beijing Academy of Food Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).