Photoageing shows histological features of chronic skin inflammation without clinical and molecular abnormalities
Photoageing shows histological features of chronic skin inflammation without clinical and molecular abnormalities
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DOI：
10.1046/j.1365-2133.2003.05456.x
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发表时间：
2003-10-01
影响因子：
10.3
通讯作者：
Nicolas, JF
中科院分区：
文献类型：
--
作者：
Bosset, S;Bonnet-Duquennoy, M;Nicolas, JF
光损伤的特征是真皮表层胶原蛋白降解和异常弹性蛋白积累。肥大细胞和巨噬细胞在光老化皮肤中数量较多，参与了这一过程。目的分析光损伤皮肤造血源性浸润细胞的表型。方法对8例接受面部拉皮手术的健康受试者进行长期日晒皮肤（耳前）和对照日晒皮肤（耳后）的修复。结果组织学分析显示，日晒皮肤比未日晒皮肤有更多的浸润性单核细胞。在日晒部位的毛囊周围弹性溶解区周围发现细胞浸润，而在日晒样品中，血管周围的毛囊间真皮和毛囊周围发现细胞浸润。免疫组织化学分析显示，与防晒样品相比，暴露在阳光下的真皮中肥大细胞、巨噬细胞和CD4+ CD45RO+ T细胞数量增加，暴露在阳光下的表皮中CD1a+树突状细胞数量增加。因此，光老化表现出慢性皮肤炎症的组织学特征。然而，没有观察到炎症的分子迹象，我们甚至发现与防晒部位相比，暴露在阳光下的白细胞介素-1 β mRNA的表达减少。此外，患者的皮肤看起来正常，没有表现出任何临床炎症。综上所述，这些数据表明，慢性紫外线照射诱导先天免疫细胞的改变，这些细胞在阳光照射的皮肤中被招募而不被激活。
Background Photodamage is characterized by degradation of collagen and accumulation of abnormal elastin in the superficial dermis. Mast cells and macrophages, which are found in higher numbers in photoaged skin, have been implicated in this process.Objectives To analyse the phenotype of haematopoietic-derived infiltrating cells in photodamaged skin.Methods Chronically sun-exposed (preauricular) and control sun-protected (postauricular) skin was recovered from eight healthy subjects undergoing cosmetic surgery (facial lifting).Results Histological analysis showed that sun-exposed skin harboured more infiltrating mononuclear cells than sun-protected skin. Cellular infiltrates were found at the periphery of areas of elastolysis around hair follicles in sun-exposed sites, whereas they were found in the interfollicular dermis around blood vessels and around hair follicles in sun-protected samples. Immunohistochemical analysis revealed an increased number of mast cells, macrophages and CD4+ CD45RO+ T cells in sun-exposed dermis as well as a higher number of CD1a+ dendritic cells in sun-exposed epidermis, compared with the sun-protected samples. Thus photoageing displays histological features of chronic skin inflammation. However, no molecular sign of inflammation was observed and we even found a decreased expression of interleukin-1beta mRNA in sun-exposed compared with sun-protected sites. Furthermore, the patients' skin looked normal and did not display any clinical inflammation.Conclusions Collectively, these data show that chronic ultraviolet irradiation induces alterations of innate immune cells which are recruited in sun-exposed skin without being activated.