Systematic analysis of the p53-related microRNAs in breast cancer revealing their essential roles in the cell cycle.
Systematic analysis of the p53-related microRNAs in breast cancer revealing their essential roles in the cell cycle.
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DOI：
10.3892/ol.2015.3751
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发表时间：
2015-12
期刊：
影响因子：
2.9
通讯作者：
Zhou J
中科院分区：
文献类型：
--
作者：
Zhou E;Hui NA;Shu M;Wu B;Zhou J
已发现许多miRNAs参与了P53信号通路的调控。相反，p53调节microRNAs(MiRNAs)的转录或加工。鉴于p53和miRNAs之间的关联存在复杂性，以及关于p53和miRNAs之间相互作用的文献数量迅速增加，本研究使用基于文献的发现方法-自然语言处理-系统地分析了乳腺癌中miRNAs和p53之间的关联。共有22个miRNAs与P53相关。接下来，使用三个流行的在线工具(PicTar、Miranda和TargetScan)来预测每个miRNA的靶标，并通过实验验证了特定的靶标。基因本体论注释和网络分析表明，与p53相关的miRNAs的大部分靶标在细胞周期过程中都得到了丰富。这些结果表明，除了调控细胞周期相关基因的转录外，P53还通过miRNAs间接调节细胞周期。
Numerous miRNAs have been found to be involved in the regulation of the p53 signaling pathway. Conversely, p53 regulates the transcription or processing of microRNAs (miRNAs). Given that complexities in the association between p53 and miRNAs exist, and due to the rapidly increasing amount of literature regarding the interactions between p53 and miRNAs, the present study systematically analyzed the associations between miRNAs and p53 in breast cancer using a literature-based discovery approach, natural language processing. A total of 22 miRNAs were found to be associated with p53. Next, three popular online tools (PicTar, miRanda and TargetScan) were used to predict the targets of each miRNA, and certain targets were validated by experiments. Gene Ontology annotation and network analysis demonstrated that the majority of the targets of the p53-related miRNAs were enriched in the cell cycle process. These results suggest that, in addition to regulating the transcription of cell cycle-related genes, p53 also indirectly modulates the cell cycle via miRNAs.