Dietary supplementation with N-carbamylglutamate increases the expression of intestinal amino acid transporters in weaned Huanjiang mini-pig piglets
Dietary supplementation with N-carbamylglutamate increases the expression of intestinal amino acid transporters in weaned Huanjiang mini-pig piglets
复制标题
日粮添加N-氨甲酰谷氨酸可增加断奶环江小型猪仔猪肠道氨基酸转运蛋白的表达
DOI：
10.2527/jas.2012-5795
复制
发表时间：
2013-06-01
影响因子：
3.3
通讯作者：
Yin, Y. L.
中科院分区：
文献类型：
--
作者：
Yang, H. S.;Fu, D. Z.;Yin, Y. L.
断奶与仔猪肠道吸收能力降低有关。本研究表明，饲粮中添加n -氨甲酰谷氨酸（NCG）可提高断奶仔猪的生长性能，改善肠道功能。本研究旨在验证饲粮中添加NCG可能通过调节肠道营养转运蛋白的表达，从而促进营养物质的吸收，从而提高断奶仔猪的生长性能。选用24头21日龄断奶（平均体重3.17 +/- 0.21 kg）的环江迷你猪仔猪，随机分为基础饲粮和基础饲粮中添加0.1% NCG的2个饲粮处理，每处理6个栏，每栏1公1母，试验期14 d。第14天，每个猪圈随机抽取1头仔猪进行血液和组织取样。饲粮中添加NCG提高了环江迷你猪断奶仔猪的生长率和饲料利用效率（P < 0.05）。添加ncg提高了空肠Slc6a19、Slc7a9和Slc1a1 mRNA表达水平（P < 0.05），以及ASCT2、B(0)AT1、B(0,+)AT、y(+)LAT1和EAAC1蛋白丰度（P < 0.05）。添加NCG对血浆低密度脂蛋白、氨、尿素氮、AA含量及碱性磷酸酶活性均有显著影响（P < 0.05）。综上所述，饲粮中添加NCG可能通过增加肠道中AA转运体的表达来改善断奶仔猪肠道吸收功能。
Weaning is associated with reduced intestinal absorptive capacity in piglets. Our previous study indicated that dietary supplementation with N-carbamylglutamate (NCG) enhanced growth performance and improved intestinal function in weaned piglets. The present study was conducted to test the hypothesis that dietary supplementation with NCG may increase the growth performance of weaned piglets by regulating the expression of intestinal nutrient transporters, thus enhancing nutrient absorption. Twenty-four Huanjiang mini-pig piglets weaned at 21 d of age (3.17 +/- 0.21 kg average BW) were randomly assigned to 2 dietary treatments consisting of a basal diet and the basal diet with 0.1% NCG supplementation for a 14-d period with 6 pens per treatment and 1 male and 1 female per pen. On d 14, 1 piglet was randomly selected from each pen for blood and tissue sampling. Dietary NCG supplementation enhanced (P < 0.05) growth rate and the efficiency of feed use in weaned Huanjiang mini-pig piglets. The NCG-supplemented diet increased (P < 0.05) mRNA expression levels of Slc6a19, Slc7a9, and Slc1a1 and the protein abundance of ASCT2, B(0)AT1, b(0,+)AT, y(+)LAT1, and EAAC1 in the jejunum. Furthermore, the contents of low density lipoprotein, ammonia, urea nitrogen, and AA as well as the activity of alkaline phosphatase in plasma were all altered (P < 0.05) by supplementation with NCG. These findings indicate that dietary supplementation with NCG may improve intestinal absorptive function in weaned piglets by increasing the expression of AA transporters in the intestine.