Differential expression profiles of microRNAs as potential biomarkers for the early diagnosis of esophageal squamous cell carcinoma
Differential expression profiles of microRNAs as potential biomarkers for the early diagnosis of esophageal squamous cell carcinoma
复制标题
microRNA的差异表达谱作为食管鳞状细胞癌早期诊断的潜在生物标志物
DOI：
10.3892/or.2012.2105
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发表时间：
2013-01-01
期刊：
影响因子：
4.2
通讯作者：
Yin, Lihong
中科院分区：
文献类型：
--
作者：
Yang, Miao;Liu, Ran;Yin, Lihong
食道鳞状细胞癌(ESCC)是世界范围内最致命的恶性肿瘤之一。为了减少该病的高发病率和死亡率，迫切需要敏感和特异的生物标志物进行早期检测。肿瘤特异性microRNAs(MiRNAs)似乎是癌症早期诊断和治疗的潜在生物标志物。本研究利用miRNA微阵列技术检测肿瘤组织和癌旁组织中差异表达的miRNAs。利用茎环实时逆转录聚合酶链式反应对芯片分析中发现的候选miRNAs进行验证。结果显示，hsa-miR-338-3p、hsa-miR-218和hsa-miR-139-5p在肿瘤组织中表达下调，而hsa-miR-183、hsa-miR-574-5p、hsa-miR-21*和hsa-miR-601在肿瘤组织中表达上调。多元回归分析显示，hsa-miR-338-3p、hsa-miR-139-5p、hsa-miR-5p和hsa-miR-601的异常表达增加了食道癌的发生风险。此外，我们还发现在大量饮酒的患者中hsa-miR-21*显著升高。因此，食管癌组织中存在一组差异表达的miRNAs，这些miRNAs可能与食管癌的发生发展有关。食管鳞癌中miRNAs的差异表达谱可能成为早期筛查高危人群和早期发现的生物标志物。
Esophageal squamous cell carcinoma (ESCC) is one of the most lethal malignancies worldwide. To reduce the high morbidity and mortality of the disease, sensitive and specific biomarkers for early detection are urgently needed. Tumor-specific microRNAs (miRNAs) seem to be potential biomarkers for the early diagnosis and treatment of cancer. In this study, differentially expressed miRNAs in tumor tissues and adjacent non-tumor tissues were detected by miRNA microarrays. Stem-loop real-time reverse transcription PCR was conducted to verify the candidate miRNAs discovered by microarray analysis. The data showed that hsa-miR-338-3p, hsa-miR-218 and hsa-miR-139-5p were downregulated in tumor tissues compared with adjacent non-tumor tissues, while hsa-miR-183, hsa-miR-574-5p, hsa-miR-21* and hsa-miR-601 were upregulated in tumor tissues. Multiple regression analysis revealed the aberrant expression of hsa-miR-338-3p, hsa-miR-139-5p, hsa-miR-574-5p and hsa-miR-601 increased the risk of esophageal cancer. Furthermore, we found hsa-miR-21* was significantly increased in heavy drinking patients. Therefore, there is a set of differentially expressed miRNAs in esophageal cancer which may be associated with the incidence and development of ESCC. Differential expression profiles of miRNAs in ESCC may be promising biomarkers for the early screening of high-risk populations and early detection.