ESTABLISHMENT OF HUMAN MICROGLIAL CELL-LINES AFTER TRANSFECTION OF PRIMARY CULTURES OF EMBRYONIC MICROGLIAL CELLS WITH THE SV40 LARGE T-ANTIGEN
ESTABLISHMENT OF HUMAN MICROGLIAL CELL-LINES AFTER TRANSFECTION OF PRIMARY CULTURES OF EMBRYONIC MICROGLIAL CELLS WITH THE SV40 LARGE T-ANTIGEN
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DOI：
10.1016/0304-3940(94)11792-h
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发表时间：
1995-08-04
影响因子：
2.5
通讯作者：
TARDIEU, M
中科院分区：
文献类型：
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作者：
JANABI, N;PEUDENIER, S;TARDIEU, M
用编码SV40大T抗原的质粒转染人胚胎脑源性巨噬细胞富集培养物，获得4个连续的人小胶质细胞细胞系。转化后的细胞具有粘附性和胞质内非特异性酯酶活性的巨噬特性。它们可以吞噬酶酶颗粒，但吞噬活性较低。它们表达几种巨噬抗原，但不表达单核细胞标志物CD14、CD4、CD68/Ki-M6和CD11c。经干扰素- γ活化后，细胞可活化表达ⅱ类主要组织相容性复合体抗原，最终细胞自发产生白细胞介素-6，经白细胞介素-1 - α刺激后，白细胞介素-6的产生进一步增加。
Four continuous cell lines of human microglial cells were obtained by transfection of enriched cultures of human embryonic brain derived macrophages with a plasmid encoding for the large T antigen of SV40. The transformed cells had the macrophagic characteristics of adherence and intra-cytoplasmic non-specific esterase activity. They could phagocytize zymosan particles but the phagocytic activity remained low. They expressed several macrophagic antigens but not the monocytic markers CD14, CD4, CD68/Ki-M6 and CD11c. The cells could be activated to express class II major histocompatibility complex antigens after interferon-gamma activation, Finally, interleukin-6 was produced spontaneously by the cells and this production was further increased after interleukin-1 alpha stimulation.