A Fluorescent Probe for Imaging p53–MDM2 Protein–Protein Interaction
A Fluorescent Probe for Imaging p53–MDM2 Protein–Protein Interaction
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DOI：
10.1111/cbdd.12434
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发表时间：
2015-04
影响因子：
3
通讯作者：
Zhenzhen Liu;Zhenyuan Miao;Jin Li;Kun Fang;Chunlin Zhuang;Lupei Du;C. Sheng;Minyong Li
中科院分区：
文献类型：
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作者：
Zhenzhen Liu;Zhenyuan Miao;Jin Li;Kun Fang;Chunlin Zhuang;Lupei Du;C. Sheng;Minyong Li
在本文中，我们描述了一种基于环境敏感荧光开启机制的无洗涤小分子荧光探针，用于检测和成像p53-MDM2蛋白-蛋白相互作用。经过广泛的生物学检验，该探针L1在体外和细胞内均表现出实际活性和选择性。
In this article, we describe a no‐wash small‐molecule fluorescent probe for detecting and imaging p53–MDM2 protein–protein interaction based on an environment‐sensitive fluorescent turn‐on mechanism. After extensive biological examination, this probe L1 exhibited practical activity and selectivity in vitro and in cellulo.