Ginsenosides Rg5 and Rh3 protect scopolamine-induced memory deficits in mice
Ginsenosides Rg5 and Rh3 protect scopolamine-induced memory deficits in mice
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DOI：
10.1016/j.jep.2012.12.047
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发表时间：
2013-03-07
影响因子：
5.4
通讯作者：
Kim, Dong-Hyun
中科院分区：
文献类型：
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作者：
Kim, Eun-Jin;Jung, Il-Hoon;Kim, Dong-Hyun
民族药理相关性：人参是五加科人参属植物，传统上被用作治疗癌症、炎症、应激和衰老的药物。研究目的：探讨人参(人参根)的主要成分人参皂苷Rg5和Rg3是否具有保护记忆障碍的作用。材料和方法：从加热人参中分别提取人参皂苷Rh3和Rg5。然后通过被动回避、Y迷宫和Morris水迷宫实验研究它们对小鼠记忆损伤的保护作用。结果：人参皂苷Rg5或Rh3能明显延长东莨菪碱所致的小鼠被动回避潜伏期。人参皂苷Rg5或Rh3可显著逆转东莨菪碱所致的Y迷宫自发改变。人参皂苷Rg5或Rh3(10 mg/kg)能显著缩短Morris水迷宫实验最后一天东莨菪碱引起的小鼠逃避潜伏期。人参皂苷Rg5和Rh3对乙酰胆碱酯酶活性的抑制作用呈剂量依赖性，IC50值分别为18.4和10.2 mU M。人参皂苷Rh3的抑制作用与多奈哌齐相当(IC50=9.9mU·M)。这些人参皂苷还能逆转东莨菪碱降低的海马脑源性神经营养因子(BDNF)表达和cAMP反应元件结合蛋白(CREB)的磷酸化。结论：人参皂苷Rg5及其代谢产物人参皂苷Rh3可能通过抑制AChE活性，增加BDNF表达和CREB活性，对记忆障碍起到保护作用。(C)爱思唯尔爱尔兰有限公司出版的2013年。
Ethnopharmacological relevance: Panax ginseng (family Araliaceae) is traditionally used as a remedy for cancer, inflammation, stress and aging.Aim of study: To explore whether ginsenosides Rg5 and Rh3, the main constituents of heat-processed ginseng (the root of Panax ginseng), could protect memory deficit.Materials and methods: We isolated ginsenosides Rh3 and Rg5 from heated-processed ginseng treated with and without human feces, respectively. Then we investigated their protective effects on memory impairment using the passive avoidance, Y-maze and Morris water maze tasks in mice. Memory deficit was induced in mice by the intraperitoneal injection of scopolamine.Results: Ginsenosides Rg5 or Rh3 increased the latency time reduced by scopolamine in passive avoidance test. Treatment with ginsenoside Rg5 or Rh3 significantly reversed the lowered spontaneous alteration induced by scopolamine in Y-maze task. Ginsenoisde Rg5 or Rh3 (10 mg/kg) significantly shortened the escape latencies prolonged by treatment with scopolamine on the last day of training trial sessions in Morris water maze task. Furthermore, ginsenosides Rg5 and Rh3 inhibited acetylcholinesterase activity in a dose-dependent manner, with IC50 values of 18.4 and 10.2 mu M, respectively. The inhibitory potency of ginsenoside Rh3 is comparable with that of donepezil (IC50=9.9 mu M). These ginsenosides also reversed hippocampal brain-derived neurotrophic factor (BDNF) expression and cAMP response element-binding protein (CREB) phospholylation reduced by scopolamine. Of them, ginsenoside Rh3 more potently protected memory deficit.Conclusions: Ginsenoside Rg5 and its metabolite ginsenoside Rh3 may protect memory deficit by inhibiting AChE activity and increasing BDNF expression and CREB activation. (C) 2013 Published by Elsevier Ireland Ltd.