Regional differences in normal and cancer-associated glycoconjugates of the human colon.
Regional differences in normal and cancer-associated glycoconjugates of the human colon.
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DOI：
10.1093/jnci/75.2.249
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发表时间：
1985-08
期刊：
影响因子：
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通讯作者：
R. Bresalier;C. Boland;Y. Kim
中科院分区：
文献类型：
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作者：
R. Bresalier;C. Boland;Y. Kim
由于结肠上皮固有的区域差异可能决定来自不同部位的肿瘤的生物学行为，并且由于结肠上皮细胞分泌的粘蛋白可能反映细胞分化状态，因此使用荧光素异硫氰酸酯偶联凝集素和荧光显微镜分析了正常胎儿和成人粘膜以及近端和远端结肠癌症的结肠杯状细胞粘蛋白。在成人近端结肠中，只有隐窝上部的杯状细胞粘蛋白被凝集素Dolichos biflorus凝集素（DBA）特异性标记，而这种梯度在远端逐渐消失；乙状结肠和直肠上、下隐窝的粘蛋白均匀地束缚着标签。在小于22周龄的胎儿中，DBA仅与远端结肠隐窝的粘蛋白结合。12例癌症中有7例（58%）起源于近端结肠结合的DBA，而23例中只有2例（9%）来自远端结肠结合这种凝集素（P < 0.005）。Logistic回归分析提示，这一差异可能反映了近端结肠肿瘤较大的发生。欧洲浮藻凝集素（UEA-I）与非肿瘤性粘膜结合的区域差异与其他研究结果相似；在成人中，主要的结合发生在近端结肠。UEA-I与近端结肠肿瘤（12例中有8例，66.6%）和远端结肠肿瘤（23例中有11例，48%）的结合没有差异（P = 0.48）。这些发现可能反映了人类结肠黏液中正常和肿瘤相关碳水化合物结构的区域差异。
Because inherent regional differences in colonic epithelium may determine the biologic behavior of tumors originating from different sites, and inasmuch as colonic epithelial cells secrete mucins that may reflect the state of cell differentiation, colonic goblet cell mucin was analyzed with the use of fluorescein isothiocyanate-conjugated lectins and fluorescence microscopy in normal fetal and adult mucosa and in cancers of the proximal and distal colon. In the adult proximal colon only the goblet cell mucin in the upper portion of the crypts was specifically labeled by the lectin Dolichos biflorus agglutinin (DBA), whereas this gradient was progressively lost distally; mucin in the upper and lower crypts of the sigmoid colon and rectum bound the label uniformly. In fetuses less than 22 weeks of age, DBA bound only to mucin in the crypts of the distal colon. Seven of 12 (58%) cancers originating from the proximal colon bound DBA, whereas only 2 of 23 (9%) from the distal colon bound this lectin (P less than .005). Logistic regression analysis suggested that this difference may reflect the occurrence of larger tumors in the proximal colon. Regional differences in the binding of Ulex europaeus agglutinin (UEA-I) to nonneoplastic mucosa was similar to that found by others; predominant binding occurred in the proximal colon in the adult. No difference was noted for UEA-I binding to tumors of the proximal (8 of 12; 66.6%) versus distal (11 of 23; 48%) colon (P = .48). These findings may reflect regional differences in normal and tumor-related carbohydrate structures in mucin of the human colon.