Uptake and transport of positron-emitting tracer (F-18) in plants
Uptake and transport of positron-emitting tracer (F-18) in plants
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DOI：
10.1016/s0969-8043(97)00117-6
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发表时间：
1997-08-01
影响因子：
1.6
通讯作者：
Sekine, T
中科院分区：
文献类型：
--
作者：
Kume, T;Matsuhashi, S;Sekine, T
正电子发射示踪成像系统是一种特殊的观测装置，它动态地跟踪正电子发射同位素在植物体内的传输，以观察植物的损伤和恢复功能。在该系统中，来自正电子发射器的湮灭伽马射线由两个平面探测器（5x 6cm（2））探测。含钙的水。将5 MBq/ml的F-18注入大豆的切割茎中2 min，然后记录30-50 min的示踪剂活性图像。当在测量前切割叶柄附近的叶的中脉时，受损叶中的活性降低，但即使在顶端也能检测到。这一结果表明，受损叶片恢复了通过叶片的水分吸收。未辐照植株叶片中的示踪剂活性在10 min内达到最大值，而100戈伊辐照植株叶片中的示踪剂活性在25 min后达到最大值，辐照植株叶片中的示踪剂活性在30 min后低于未辐照植株。在豆类的情况下，有一个未照射和照射样品之间的F-18标记的水的吸收行为的差异。这些结果表明，该系统是有效的，以观察含正电子发射示踪剂的水的吸收和运输的植物损伤和恢复功能的研究。(C)1997年爱思唯尔科学有限公司
The transport of a positron-emitting isotope introduced into a plant was dynamically followed by a special observation apparatus called 'Positron-Emitting Tracer Imaging System' to observe the damage and recovery functions of plants in vivo. in the system, annihilation gamma-rays from the positron emitter are detected with two planar detectors (5 x 6 cm(2)). The water containing ca. 5 MBq/ml of F-18 was fed to the cut stem of soybean for 2 min and then the images of tracer activity were recorded for 30-50 min. When the midrib of a leaf near the petiole was cut just before measurement, the activity in the injured leaf was decreased but detected even at the apex. This result suggests that the damaged leaf recovered the uptake of water through the lamina. Maximum tracer activities in leaves of unirradiated plant were observed within 10 min, whereas those of irradiated plant at 100 Gy were observed after over 25 min. The final activity of irradiated plant after 30 min was lower than that of unirradiated plant. In case of beans, there was a difference in the absorption behavior of the F-18-labeled water between unirradiated and irradiated samples. These results show that the system is effective to observe the uptake and transportation of water containing positron emitting tracer for the study of damage and recovery functions of plants. (C) 1997 Elsevier Science Ltd.