Hepatoprotective Effect of Syringic Acid and Vanillic Acid on CCl4-Induced Liver Injury
Hepatoprotective Effect of Syringic Acid and Vanillic Acid on CCl4-Induced Liver Injury
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DOI：
10.1248/bpb.33.983
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发表时间：
2010-06-01
影响因子：
2
通讯作者：
Yagi, Kiyohito
中科院分区：
文献类型：
--
作者：
Itoh, Ayano;Isoda, Katsuhiro;Yagi, Kiyohito
食用菌香菇菌丝体可在含有木质素的固体基质中培养，其热水浸出物(L.E.M.)是一种商业上可用的营养补充剂。在培养过程中，香菇菌丝体分泌的木质素降解过氧化物酶产生丁香酸和香草酸等酚类化合物。由于这些化合物具有清除自由基的活性，我们研究了它们对CCl4诱导的肝损伤小鼠氧化应激的保护作用。我们研究了丁香酸和香草酸对CCl4诱导的小鼠慢性肝损伤的保护作用。CCl4腹腔注射可引起血清天冬氨酸转氨酶(AST)和丙氨酸氨基转移酶(ALT)水平升高。静脉注射丁香酸和香草酸可显著降低转氨酶水平。四周的CCl4治疗导致足够多的胶原纤维沉积。Azan染色的肝切片检查显示，丁香酸和香草酸明显抑制胶原沉积，并显著降低肝组织羟脯氨酸含量，这是肝纤维化的定量标志。这两个化合物都抑制了培养的肝星状细胞的激活，而培养的肝星状细胞在肝纤维化形成中起着核心作用，并维持了肝细胞的活力。提示丁香酸和香草酸对慢性肝损伤的肝纤维化有抑制作用。
The mycelia of the edible mushroom Lentinula edodes can be cultured in solid medium containing lignin, and the hot-water extracts (L.E.M.) is commercially available as a nutritional supplement. During the cultivation, phenolic compounds, such as syringic acid and vanillic acid, were produced by lignin-degrading peroxidase secreted from L. edodes mycelia. Since these compounds have radical scavenging activity, we examined their protective effect on oxidative stress in mice with CCl4-induced liver injury. We examined the hepatoprotective effect of syringic acid and vanillic acid on CCl4-induced chronic liver injury in mice. The injection of CCl4 into the peritoneal cavity caused an increase in the serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) levels. The intravenous administration of syringic acid and vanillic acid significantly decreased the levels of the transaminases. Four weeks of CCl4 treatment caused a sufficiently excessive deposition of collagen fibrils. An examination of Azan-stained liver sections revealed that syringic acid and vanillic acid obviously suppressed collagen accumulation and significantly decreased the hepatic hydroxyproline content, which is the quantitative marker of fibrosis. Both of these compounds inhibited the activation of cultured hepatic stellate cells, which play a central role in liver fibrogenesis, and maintained hepatocyte viability. These data suggest that the administration of syringic acid and vanillic acid could suppress hepatic fibrosis in chronic liver injury.