A new tospovirus causing chlorotic ringspot on Hippeastrum sp in China
A new tospovirus causing chlorotic ringspot on Hippeastrum sp in China
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一种引起中国朱顶红褪绿环斑的新拓扑病毒
DOI：
10.1007/s11262-012-0873-z
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发表时间：
2013-06-01
期刊：
影响因子：
1.6
通讯作者：
Zhang, Z. K.
中科院分区：
文献类型：
--
作者：
Dong, J. H.;Yin, Y. Y.;Zhang, Z. K.
从云南省朱顶红属一种表现坏死和褪绿环斑症状的植物中分离到一种新的番茄斑萎病毒，命名为HCRV 2007-ZDH。该病毒分离物的特征在于基于颗粒形态和RNA序列分析。在受感染植物的汁液和细胞中观察到测量约70-100 nm的准球形包膜颗粒，这是番茄斑萎病毒的典型颗粒。通过对该分离物的机械侵染研究，证实2007-ZDH是朱顶红黄化环斑病的病原。2007-ZDH的S RNA全长为2,744个核苷酸，与番茄黄环病毒（TYRV）分离物番茄（AY 686718）的核苷酸同源性为74.4%。SRNA编码一个非结构蛋白（NSs）（444 aa，50.4 kDa）和一个核衣壳蛋白（N）（273 aa，30.1 kDa），与TYRV、IYSV和PolRSV的NSs蛋白的氨基酸同源性分别为78.6%、76.3%和74.9%。N蛋白与PolRSV、TYRV和IYSV的氨基酸同源性分别为86.1%、84.7%和70.0%。这些结果表明，褪绿环斑病毒属于番茄斑病毒属的一个新种，建议将其命名为朱顶红褪绿环斑病毒（HCRV）。
A new tospovirus, HCRV 2007-ZDH, was isolated from a Hippeastrum sp. plant displaying necrotic and chlorotic ringspot symptoms in Yunnan province. This virus isolate was characterized based on particle morphology and RNA sequences analyses. Quasi-spherical, enveloped particles measuring about 70-100 nm, typical of tospoviruses, were observed in sap and cells of the infected plants. Transmission studies by inoculating this isolate mechanically to Hippeastrum sp. confirmed that 2007-ZDH is the causal agent of the chlorotic ringspot disease of Hippeastrum sp. The complete sequence of S RNA of 2007-ZDH was 2,744 nucleotides in length, sharing 74.4 % nucleotide identity with Tomato yellow ring virus (TYRV) isolate tomato (AY686718). The S RNA encoded a non-structural protein (NSs) (444 aa, 50.4 kDa) and the nucleocapsid (N) protein (273 aa, 30.1 kDa).The deduced NSs protein shared amino acid identities of 78.6, 76.3, and 74.9 % with that of TYRV, IYSV, and PolRSV, respectively. The deduced N protein shared amino acid identities of 86.1, 84.7, and 70.0 % with that of PolRSV, TYRV, and IYSV, respectively. These results suggest that the chlorotic ringspot virus belongs to a new tospovirus species, for which the name Hippeastrum chlorotic ringspot virus (HCRV) is proposed.