Molecular decoys: ligand-binding recombinant proteins protect mice from curarimimetic neurotoxins.
Molecular decoys: ligand-binding recombinant proteins protect mice from curarimimetic neurotoxins.
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分子诱饵：配体结合重组蛋白保护小鼠免受类箭毒神经毒素的侵害。
DOI：
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发表时间：
1988
影响因子：
11.1
通讯作者：
A. Aronheim
中科院分区：
文献类型：
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作者：
J. Gershoni;A. Aronheim
烟碱乙酰胆碱受体的模拟配体结合位点在体外结合 d-管箭毒碱和 α-银环蛇毒素。将此类结合位点注射到小鼠体内可以充当体内分子诱饵，提供针对有毒配体的保护。这种分子“诱饵”假说已在超过 250 只小鼠身上进行了测试。细菌产生的胆碱能结合位点使受到类箭毒神经毒素攻击的动物的存活率提高了两倍。讨论了诱饵设计及其应用的可能考虑因素。
Mimic ligand-binding sites of the nicotinic acetylcholine receptor bind d-tubocurarine and alpha-bungarotoxin in vitro. Injection of such binding sites into mice could act as molecular decoys in vivo, providing protection against toxic ligands. This hypothesis of molecular "decoyance" has been tested in greater than 250 mice. Bacterially produced cholinergic binding sites provided a 2-fold increase in the survival rate of animals challenged with curarimimetic neurotoxins. Possible considerations for decoy designs and their applications are discussed.