[Enzyme systems of the substrate and cofactor supply of hyperlipogenesis in non-insulin-dependent diabetes].
[Enzyme systems of the substrate and cofactor supply of hyperlipogenesis in non-insulin-dependent diabetes].
复制标题
[非胰岛素依赖型糖尿病中脂肪生成过多的底物和辅因子供应的酶系统]。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
A. Moiseenok
中科院分区：
文献类型：
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作者：
I. Obrosova;A. Efimov;N. N. Velikii;T. Zimatkina;A. Moiseenok
以非胰岛素依赖型糖尿病模型C57Bl/Ks J小鼠（db/db）为实验对象，研究了由底物（乙酰辅酶a）和辅助因子（NADPH和ATP）供应的肝脏高脂生成酶系统。乙酰辅酶a合成酶活性升高，丙酮酸脱氢酶复合物活性与对照无明显差异，atp -柠檬酸裂解酶活性降低。非胰岛素依赖性糖尿病的高脂生成是由于细胞能量供应的激活，表现为2-氧戊二酸脱氢酶活性的增强和ATP水平的升高，以及NADPH供应和利用的活性比的变化和脂肪酸合成酶变构效应物果糖-1,6-二磷酸的增加，导致酶活性的增加，使NADPH作为脂肪生成的还原等效物的利用潜力更大。
Enzymatic systems of hepatic hyperlipogenesis supply by substrate (acetyl-CoA) and cofactors (NADPH and ATP) were studied in experiments on diabetic C57Bl/Ks J mice (db/db) that served as a model of non-insulin dependent diabetes. The rise in acetyl-CoA synthetase activity catalyzing the primary step of lipogenesis from acetate has been found, while pyruvate dehydrogenase complex activity did not differ from the control and ATP-citrate lyase activity was lowered. Hyperlipogenesis in non-insulin dependent diabetes was induced by the activation of cellular energy supply revealed in enhanced 2-oxoglutarate dehydrogenase activity and elevated ATP level, as well as changes in the activity ratio of NADPH supply and utilization and the rise in fructose-1,6-diphosphate, allosteric effector of fatty acid synthetase, which resulted in the increase of the enzyme activity and created wider potentials of NADPH utilization as a reducing equivalent in lipogenesis.