Generation of a tissue-engineered thymic organoid.
Generation of a tissue-engineered thymic organoid.
复制标题
组织工程胸腺类器官的生成。
DOI：
10.1007/978-1-59745-395-0_9
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
Poznansky,MarkC
中科院分区：
文献类型：
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作者：
Vianello,Fabrizio;Poznansky,MarkC
胸腺微环境为产生功能多样的人T细胞群体提供了必要的支持。特别是，三维（3D）胸腺结构有助于关键的细胞-细胞相互作用。我们报告说，胸腺基质，排列在一个合成的3D矩阵，支持从造血前体细胞的功能性人类T细胞的发展。新产生的T细胞含有T细胞受体切除环，并且完全成熟和功能性。因此，T细胞祖细胞与胸腺基质的共培养可用于产生新功能的和多样化的T细胞群。这种新的组织工程胸腺系统具有用于研究T淋巴细胞生成和自身耐受性的生物学应用以及潜在的治疗应用，包括免疫功能低下患者的免疫重建和接受组织或器官移植的个体中耐受性的诱导。
The thymic microenvironment provides essential support for the generation of a functional and diverse population of human T cells. In particular, the three-dimensional (3D) thymic architecture contributes to critical cell-cell interactions. We report that thymic stroma, arrayed on a synthetic 3D matrix, supports the development of functional human T cells from hematopoietic precursor cells. Newly generated T cells contain T-cell receptor excision circles and are both fully mature and functional. The coculture of T-cell progenitors with thymic stroma can thus be used to generatede novofunctional and diverse T-cell populations. This novel tissue engineered thymic system has biological applications for the study of T-lymphopoiesis and self-tolerance as well as potential therapeutic applications including the immune reconstitution of immunocompromised patients and the induction of tolerance in individuals receiving tissue or organ transplants.