The homology gene BtDnmt1 is Essential for Temperature Tolerance in Invasive Bemisia tabaci Mediterranean Cryptic Species.
The homology gene BtDnmt1 is Essential for Temperature Tolerance in Invasive Bemisia tabaci Mediterranean Cryptic Species.
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同源基因 BtDnmt1 对于地中海隐秘物种烟粉虱的温度耐受性至关重要
DOI：
10.1038/s41598-017-03373-w
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发表时间：
2017-06-08
影响因子：
4.6
通讯作者：
Hong XY
中科院分区：
文献类型：
--
作者：
Dai TM;Lü ZC;Liu WX;Wan FH;Hong XY
地中海烟粉虱(MED)由于具有很强的生态适应性，特别是对温度胁迫的抵抗力强，一直在迅速入侵世界大部分地区。表观遗传机制在调节生态可塑性方面起着重要作用。特别是，DNA甲基化一直是试图了解表型可塑性机制的重点。温度和DNA甲基化之间的关系以及它如何影响入侵昆虫的适应能力仍然是未知的。为了研究DNA甲基转移酶1(Dnmt1)在MED中的抗温作用，我们克隆并测序了BtDnmt1同源基因，并鉴定了其在不同温度条件下的功能。MED BtDnmt1同源基因全长为5,958个碱基，有一个4,287个碱基的开放阅读框，编码1,428个氨基酸。给予dsRNA后，BtDnmt1mRNA的表达水平明显下调。此外，在摄食dsBtDnmt1后，MED成虫在温度应激条件下的死亡率显著高于对照组，表明MED BtDnmt1同源基因在MED的耐温能力中起着至关重要的作用。我们的数据提高了我们对温度抗性和温度适应机制的理解，这些机制允许这种外来物种成功地入侵和殖民各种环境。
The Bemisia tabaci Mediterranean (MED) cryptic species has been rapidly invading most parts of the world owing to its strong ecological adaptability, particularly its strong resistance to temperature stress. Epigenetic mechanisms play important roles in mediating ecological plasticity. In particular, DNA methylation has been the focus of attempts to understand the mechanism of phenotypic plasticity. The relationship between temperature and DNA methylation and how it affects the adaptability of invasive insects remain unknown. To investigate the temperature resistance role of DNA methyltransferase 1 (Dnmt1) in MED, we cloned and sequenced BtDnmt1 homology and identified its functions under various temperature conditions. The full-length cDNA of MED BtDnmt1 homology was 5,958 bp and has a 4,287 bp open reading frame that encodes a 1,428-amino-acid protein. BtDnmt1 mRNA expression levels were significantly down-regulated after feeding with dsRNA. Furthermore, after feeding with dsBtDnmt1, the MED adults exhibited significantly higher mortality under temperature stress conditions than the controls, suggesting that MED BtDnmt1 homology plays an essential role in the temperature tolerance capacity of MED. Our data improve our understanding of the temperature resistance and temperature adaptability mechanisms that have allowed the successful invasion and colonization of various environments by this alien species.