Multi-walled carbon nanotubes: Lack of mutagenic activity in the bacterial reverse mutation assay
Multi-walled carbon nanotubes: Lack of mutagenic activity in the bacterial reverse mutation assay
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DOI：
10.1016/j.toxlet.2008.11.007
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发表时间：
2009-02-10
影响因子：
3.5
通讯作者：
Mazzanti, Gabriela
中科院分区：
文献类型：
--
作者：
Di Sotto, Antonella;Chiaretti, Massimo;Mazzanti, Gabriela
在存在和不存在代谢活化系统S9的情况下，通过细菌回复突变试验（艾姆斯试验）对鼠伤寒沙门氏菌TA 98和TA 100菌株以及大肠杆菌WP 2 uvrA菌株评价了多壁碳纳米管（MWCNT）的致突变作用（其特征为小表面积/体积比、大直径和金属污染物小于0.1%）。进行了初步的细胞毒性试验，以确保细胞毒性不会干扰响应。多壁碳纳米管在测试的细菌细胞系统中没有致突变作用，因为它们不会显著增加回复突变菌落的数量。甚至在存在代谢活化剂的情况下也没有出现致突变活性，因此我们可以排除通过基于细胞色素的P450代谢氧化系统产生的多壁碳纳米管代谢物可能作为诱变剂。碳纳米材料似乎表现出不同的生物活性和不同的毒性，这与它们的物理化学特性、尺寸、形状、结晶度和金属痕量的存在有关，因此很难确定他们健康风险。由于遗传毒性研究的有限背景和纳米材料的职业和公众暴露的增加，目前的研究结果似乎是有用的，以扩大碳纳米管的安全知识，鉴于其可能的应用。(C)2008爱思唯尔爱尔兰有限公司保留所有权利。
The mutagenic effect of multi-walled carbon nanotubes (MWCNTs) characterised by small surface/volume ratio, high diameter and less than 0.1% of metal contaminants was evaluated by the bacterial reverse mutation assay (Ames test) on Salmonella typhimurium TA 98 and TA 100 strains, and on Escherichia coli WP2uvrA strain, in presence and in absence of the metabolic activation system S9. A preliminary cytotoxicity assay was carried out to ensure that cytotoxicity did not interfere with response.MWCNTs resulted devoid of mutagenic effect in the bacterial cellular systems tested in that they did not significantly increase the number of revertant colonies. The mutagenic activity did not even appear in presence of the metabolic activator, so we can exclude that MWCNTs metabolites, produced via cytochrome-based P450 metabolic oxidation system, may act as mutagens.Carbon nanomaterials seem to exhibit different biological activities and different toxicities in relation to their physico-chemical characteristics, size, shape, crystallinity and presence of metal traces, so it is difficult to establish their health risk. Due to the limited background of genotoxicity studies and the increased occupational and public exposure to nanomaterials, present results appear useful to extend the knowledge on the safety of carbon nanotubes in view of their possible applications. (C) 2008 Elsevier Ireland Ltd. All rights reserved.