Biofilm and multidrug resistance in uropathogenic Escherichia coli
Biofilm and multidrug resistance in uropathogenic Escherichia coli
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DOI：
10.1179/2047773215y.0000000001
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发表时间：
2015-02-01
影响因子：
3.4
通讯作者：
Chaudhary, Uma
中科院分区：
文献类型：
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作者：
Mittal, Seema;Sharma, Madhu;Chaudhary, Uma
背景：大肠杆菌被认为是尿路感染（uti）的病原体，它倾向于在膀胱粘膜衬里形成微菌落，称为生物膜。这些生物膜通过将其包裹在细胞外生化基质中，使生物体抵抗宿主免疫反应，更具毒性，并导致抗菌药物耐药性的进化。目的：了解尿路致病性大肠杆菌（UPEC）中各种毒力因子与生物膜生成的关系及抗生素的药敏模式。背景和设计：本研究于2011年1月至2011年12月在哈里亚纳邦罗塔克的p.b.d. Sharma PGIMS进行，为期一年。方法与材料：采用微滴板（MTP）法检测生物膜，检测溶血素、血凝、明胶酶、铁载体生成、血清抗性、疏水性等毒力因子。药敏试验采用改良Kirby-Bauer纸片扩散法，纸片扩散法确定ESBL、AmpC、MBL产率，采用UPEC统计分析，数据采用SPSS 17.0版本进行分析。双侧p值小于或等于0.05被认为是显著的。结果：18株（13.5%）分离株产生生物膜，以雌株多见（2次）。发现这些分离株对常用抗生素具有耐药性，为100%耐多药。结论：与非生物膜生产者相比，生物膜的产生使生物体对抗生素的耐药性和毒性更强。
Context: Escherichia coli is known as causative agent of urinary tract infections (UTIs) tends to form microcolonies in mucosa lining of urinary bladder known as biofilm. These biofilms make the organism to resist the host immune response, more virulent and lead to the evolution of antibacterial drug resistance by enclosing them in an extracellular biochemical matrix.Aims: This study was done to know the association of various virulence factors and biofilm production in uropathogenic E. coli (UPEC) and antibiotic susceptibility pattern.Settings and design: This study was conducted in Pt. B.D. Sharma PGIMS, Rohtak, Haryana during a period of 1 year from January 2011 to December 2011.Methods and material: Biofilm was detected by microtiter plate (MTP) method, and various virulence factors like hemolysin, hemagglutination, gelatinase, siderophore production, serum resistance, and hydrophobicity were detected. The antibiotic susceptibility testing was done by modified Kirby-Bauer disk diffusion and the disk diffusion method was used to confirm the ESBL, AmpC, MBL production by the UPEC statistical analysis used: The data were analyzed by using SPSS version 17.0. A two-sided P-value of less than or equal to 0.05 was considered to be significant.Results: Biofilm production was found in 18 (13.5%) isolates, more commonly in females (two times). These isolates were found to be resistant to antibiotics common in use and were 100% MDR.Conclusions: Biofilm production makes the organism to be more resistant to antibiotics and virulent as compared to non-biofilm producers.