A Molecular Titration System Coordinates Ribosomal Protein Gene Transcription with Ribosomal RNA Synthesis
A Molecular Titration System Coordinates Ribosomal Protein Gene Transcription with Ribosomal RNA Synthesis
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DOI：
10.1016/j.molcel.2016.10.003
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发表时间：
2016-11-17
期刊：
影响因子：
16
通讯作者：
Shore, David
中科院分区：
文献类型：
--
作者：
Albert, Benjamin;Knight, Britta;Shore, David
细胞生长潜力由核糖体生物发生的速度决定，这是一个复杂的过程，需要大量和协调的转录输出。在酿酒酵母中，核糖体的生物发生在转录水平上受到高度调控。虽然已经描述了协调核糖体RNA(RRNA)和核糖体蛋白基因(RPG)转录的系统的证据，但其分子机制仍然知之甚少。在这里，我们证明了RPG转录激活子Ifh1和rRNA处理因子Utp22之间的相互作用有助于协调RPG转录和rRNA的转录。我们证明了在生长抑制之后，Ifh1可以通过Utp22不依赖的机制从RPG启动子中迅速释放出来，但它的长期解离需要Utp22。我们提出了RNA聚合酶I活性抑制Utp22从RPG启动子滴定Ifh1的能力的证据，并提出动态的Ifh1-Utp22相互作用微调RPG的表达，以协调RPG和rRNA转录。
Cell growth potential is determined by the rate of ribosome biogenesis, a complex process that requires massive and coordinated transcriptional output. In the yeast Saccharomyces cerevisiae, ribosome biogenesis is highly regulated at the transcriptional level. Although evidence for a system that coordinates ribosomal RNA (rRNA) and ribosomal protein gene (RPG) transcription has been described, the molecular mechanisms remain poorly understood. Here we show that an interaction between the RPG transcriptional activator Ifh1 and the rRNA processing factor Utp22 serves to coordinate RPG transcription with that of rRNA. We demonstrate that Ifh1 is rapidly released from RPG promoters by a Utp22-independent mechanism following growth inhibition, but that its long-term dissociation requires Utp22. We present evidence that RNA polymerase I activity inhibits the ability of Utp22 to titrate Ifh1 from RPG promoters and propose that a dynamic Ifh1-Utp22 interaction fine-tunes RPG expression to coordinate RPG and rRNA transcription.