Enhanced AZIN1 RNA editing and overexpression of its regulatory enzyme ADAR1 are important prognostic biomarkers in gastric cancer
Enhanced AZIN1 RNA editing and overexpression of its regulatory enzyme ADAR1 are important prognostic biomarkers in gastric cancer
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DOI：
10.1186/s12967-018-1740-z
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发表时间：
2018-12-18
影响因子：
7.4
通讯作者：
Goel, Ajay
中科院分区：
文献类型：
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作者：
Okugawa, Yoshinaga;Toiyama, Yuji;Goel, Ajay
背景
腺苷到肌苷（A - I）的RNA编辑是由作用于RNA的腺苷脱氨酶（ADAR）催化的。近期有证据表明，抗酶抑制剂1（AZIN1）RNA的编辑正成为癌症发病机制中一种关键的表观遗传学改变。
方法
我们使用RNA编辑位点特异性定量聚合酶链反应检测方法，评估了140名患者的280份胃组织中AZIN1 RNA编辑水平及其调节因子ADAR1的表达。我们还分析了这些结果作为胃癌（GC）患者疾病生物标志物的临床意义。
结果
与匹配的正常黏膜相比，胃癌组织中AZIN1 RNA编辑水平和ADAR1表达均显著升高（P
BackgroundAdenosine-to-inosine (A-to-I) RNA editing is catalyzed by adenosine deaminases acting on RNA (ADAR) enzymes. Recent evidence suggests that RNA editing of antizyme inhibitor 1 (AZIN1) RNA is emerging as a key epigenetic alteration underlying cancer pathogenesis.MethodsWe evaluated AZIN1 RNA editing levels, and the expression of its regulator, ADAR1, in 280 gastric tissues from 140 patients, using a RNA editing site-specific quantitative polymerase chain reaction assays. We also analyzed the clinical significance of these results as disease biomarkers in gastric cancer (GC) patients.ResultsBoth AZIN1 RNA editing levels and ADAR1 expression were significantly elevated in GC tissues compared with matched normal mucosa (P