NK cells contribute to intracellular bacterial infection-mediated inhibition of allergic responses
NK cells contribute to intracellular bacterial infection-mediated inhibition of allergic responses
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DOI：
10.4049/jimmunol.180.7.4621
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发表时间：
2008-04-01
影响因子：
4.4
通讯作者：
Yang, Xi
中科院分区：
文献类型：
--
作者：
Han, Xiaobing;Fan, Yijun;Yang, Xi
为了验证卫生学假说，我们利用小鼠气道炎症模型研究了衣原体感染对OVA诱导的过敏反应的影响以及NK细胞在这一过程中的参与。我们发现既往的穆里达衣原体感染可以抑制由过敏原致敏和攻击引起的气道嗜酸性粒细胞炎症和粘液的产生。这种抑制作用与T细胞过敏原驱动的细胞因子产生模式的改变有关。我们证明NK细胞在衣原体感染后被激活，显示细胞扩增和细胞因子分泌。在OVA致敏和激发前使用anti-NK Ab在体内消耗NK细胞部分消除了衣原体感染的抑制作用，这与Th2细胞因子产生的部分恢复有关。相比之下，从感染小鼠中分离的NK细胞过继转移对过敏反应表现出明显的抑制作用，与自然感染中观察到的相似。提示NK细胞等先天免疫细胞可能在感染介导的过敏反应抑制中发挥重要作用。
To experimentally examine the hygiene hypothesis, here we studied the effect of chlamydial infection on the development of allergic responses induced by OVA and the involvement of NK cells in this process using a mouse model of airway inflammation. We found that prior Chlamydia muridarum infection can inhibit airway eosinophilic inflammation and mucus production induced by allergen sensitization and challenge. The inhibition was correlated with an alteration of allergen-driven cytokine-producing patterns of T cells. We demonstrated that NK cells were activated following chlamydial infection, showing both cell expansion and cytokine secretion. The in vivo depletion of NK cells using anti-NK Ab before OVA sensitization and challenge partially abolished the inhibitory effect of chlamydial infection, which was associated with a partial restoration of Th2 cytokine production. In contrast, the adoptive transfer of NK cells that were isolated from infected mice showed a significant inhibitory effect on allergic responses, similar to that observed in natural infection. The data suggest that the innate immune cells such as NK cells may play an important role in infection-mediated inhibition of allergic responses.