Film bulk acoustic resonators for DNA and protein detection and investigation of in vitro bacterial S-layer formation
Film bulk acoustic resonators for DNA and protein detection and investigation of in vitro bacterial S-layer formation
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DOI：
10.1016/j.sna.2009.02.021
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发表时间：
2009-11-01
影响因子：
4.6
通讯作者：
Mertig, Michael
中科院分区：
文献类型：
--
作者：
Nirschl, Martin;Blueher, Anja;Mertig, Michael
我们报告了使用薄膜体声谐振器进行DNA和蛋白质的无标记、多路生物传感的研究。所用的声学谐振器以大约800 MHz的剪切模式运行。根据测量的频率和耗散的变化，可以分别推导出生物分子膜在装置顶部电极上形成的质量和粘弹性。质量灵敏度接近2 kHz cm(2)/ng，最小可检测质量接近1 ng/cm(2)。为了证明对蛋白质吸附随时间演化的高灵敏度检测，研究了细菌表面层蛋白质在金表面的吸附动力学和重结晶。(C)2009爱思唯尔B.V.保留所有权利。
We report investigations on the use of film bulk acoustic resonators for the label-free, multiplexed biosensing of DNA and proteins. The used acoustic resonators were operated in shear mode at about 800 MHz. From the measured changes of frequency and in dissipation, the mass and the viscoelasticity of biomolecular films formed at the top electrode of the device could be derived, respectively. A mass sensitivity of similar to 2 kHz cm(2)/ng and a minimum detectable mass of similar to 1 ng/cm(2) were achieved. To demonstrate the highly sensitive detection of the time evolution of protein adsorption, the adsorption kinetics and recrystallisation of bacterial surface layer proteins on gold surfaces were investigated. (C) 2009 Elsevier B.V. All rights reserved.