KERATIN IN EPITHELIOID SARCOMA - AN IMMUNOHISTOCHEMICAL STUDY
KERATIN IN EPITHELIOID SARCOMA - AN IMMUNOHISTOCHEMICAL STUDY
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DOI：
10.1097/00000478-198406000-00004
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发表时间：
1984-01-01
影响因子：
5.6
通讯作者：
LANGLOSS, JM
中科院分区：
文献类型：
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作者：
CHASE, DR;ENZINGER, FM;LANGLOSS, JM
先前的研究人员已经在上皮样肉瘤中注意到了中等大小的丝状体，其中两名研究人员报告说这些丝状体代表波形蛋白。使用针对角蛋白的多克隆抗体和免疫过氧化物酶技术对超过 300 例病例中的 32 例进行染色。所有材料均经过福尔马林固定、石蜡包埋。 75% 的病例 (24/32) 显示阳性免疫反应性，这一特征可能有助于诊断上皮样肉瘤与结节性筋膜炎、良性和恶性纤维组织细胞瘤、恶性黑色素瘤和坏死性肉芽肿。在这些情况下，使用胰蛋白酶预消化可增强反应。免疫反应性因肿瘤而异，可能是由于福尔马林固定所致。由于滑膜肉瘤和间皮瘤也可能呈细胞角蛋白阳性，因此角蛋白免疫反应性并不局限于上皮性肿瘤，也可能发生在传统上被认为是间叶性的肿瘤中。
Intermediate-sized filaments have been noted in epithelioid sarcoma by previous investigators, 2 of whom have reported that the filaments represent vimentin. Polyclonal antibodies directed against keratin and immunoperoxidase techniques were used to stain 32 of the > 300 cases. All of the material was formalin-fixed, paraffin-embedded. Seventy-five percent of the cases (24/32) showed positive immunoreactivity, a feature that may be of diagnostic help in distinguishing epithelioid sarcoma from nodular fasciitis, benign and malignant fibrous histiocytoma, malignant melanoma, and necrotizing granuloma. In these cases, the reaction was enhanced using predigestion with trypsin. The immunoreactivity varied from tumor to tumor, perhaps due to formalin fixation. Since synovial sarcoma and mesothelioma may also be cytokeratin-positive, keratin immunoreactivity is not confined to epithelial tumors and may also occur in neoplasms traditionally regarded as mesenchymal.