Auxin-induced deoxyribonucleic acid dependent ribonucleic acid polymerase activities in mature soybean hypocotyl.
Auxin-induced deoxyribonucleic acid dependent ribonucleic acid polymerase activities in mature soybean hypocotyl.
复制标题
成熟大豆下胚轴中生长素诱导的脱氧核糖核酸依赖性核糖核酸聚合酶活性。
DOI：
10.1021/bi00567a025
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发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
Guilfoyle,TJ
中科院分区：
文献类型：
--
作者：
Guilfoyle,TJ
Tom J. Guilfoyle摘要：用人工合成的生长素、2，4-二氯苯氧乙酸处理3日龄大豆黄化幼苗时，成熟下胚轴细胞发生异常膨大和增殖。在施用生长素48小时后，核糖核酸（RNA）聚合酶I和II的水平分别增加了约10 -20倍和6倍（以鲜重组织为基础）和约3-6倍和2倍（以组织脱氧核糖核酸（DNA）为基础）。[35S]甲硫氨酸掺入RNA聚合酶亚基表明，这种RNA聚合酶水平的增加是由酶的从头合成引起的。亚基结构或模式没有改变，
Tom J. Guilfoyle abstract: When 3-day-old etiolated soybean seedlings are treated with the synthetic auxin, 2, 4-dichlorophenoxyacetic acid, cells of the mature hypocotyl swell and proliferate ab-normally. By 48 h after auxin application ribonucleic acid (RNA) polymerase I and II levels increase by about10-20-and 6-fold, respectively, on a fresh weight tissue basis and about 3-6-and 2-fold, respectively, on a tissue deoxyribo-nucleic acid (DNA) basis.[35S] Methionine incorporation into RNA polymerase subunits suggests that this increase in levels of RNA polymerases results from de novo synthesis of the enzymes. No alteration in subunit structure or patterns of