Effect of tunicamycin on molecular heterogeneity of colony stimulating factor in cultured mouse mammary carcinoma FM3A cells.
Effect of tunicamycin on molecular heterogeneity of colony stimulating factor in cultured mouse mammary carcinoma FM3A cells.
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衣霉素对培养的小鼠乳腺癌FM3A细胞集落刺激因子分子异质性的影响。
DOI：
10.1016/0006-291x(79)91896-5
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
G. Tamura
中科院分区：
文献类型：
--
作者：
D. Ayusawa;K. Isaka;T. Seno;M. Tomida;Y. Yamamoto;M. Hozumi;A. Takatsuki;G. Tamura
从培养的小鼠乳腺癌 FM3A 细胞中获得的粒细胞和巨噬细胞集落刺激因子在分子大小上表现出异质性，导致在 Sephadex G-200 柱色谱上产生的主要成分的表观分子量为 80,000，次要成分的表观分子量为 35,000。在天冬酰胺联糖基化的特异性抑制剂衣霉素存在下，集落刺激因子正常产生，并且由表观分子量为30,000的单一组分组成。这些数据表明，糖部分对于集落刺激因子的产生或活性不是必需的，并且集落刺激因子分子大小的异质性主要是由衣霉素敏感的糖基化造成的。
Granulocyte and macrophage colony stimulating factors obtained from cultured mouse mammary carcinoma FM3A cells showed heterogeneity in molecular size giving rise to a major component with an apparent molecular weight of 80,000 and a minor one with that of 35,000 on Sephadex G-200 column chromatography. In the presence of tunicamycin, a specific inhibitor of asparagine-linked glycosylation, the colony stimulating factor was produced normally and consisted of a single component with an apparent molecular weight of 30,000.These data indicate that the sugar moiety is not essential for the production or activity of colony stimulating factor and that the heterogeneity in molecular size of the colony stimulating factor mainly resulted from tunicamycin-sensitive glycosylation.