Lectin-based structural glycomics: Glycoproteomics and glycan profiling
Lectin-based structural glycomics: Glycoproteomics and glycan profiling
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DOI：
10.1023/b:glyc.0000043745.18988.a1
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发表时间：
2004-01-01
影响因子：
3
通讯作者：
Hirabayashi, J
中科院分区：
文献类型：
--
作者：
Hirabayashi, J
结构糖组学（SG）是并发糖生物学的一个基本组成部分，旨在全面阐明后基因组科学背景下的糖功能（即功能糖组学）。SG项目于2003年4月开始，将在日本经济产业省（METI）下属的NEDO（新能源和工业技术组织）的框架下持续3年。该项目的主要目的是开发高通量和强大的机器，这将大大有助于复杂聚糖的结构分析。本章主要介绍了两个主要的研究项目，即：(1)糖蛋白组学，能够对糖蛋白进行综合分析；(2)通过凝集素进行“聚糖谱分析”。对于后者，采用正面亲和色谱法作为起始工具，在“百乘百”的概念下，对100种凝集素和100种低聚糖的相互作用进行综合分析。
Structural glycomics (SG) plays a fundamental part of concurrent glycobiology aiming at comprehensive elucidation of glycan functions (i.e., functional glycomics) in the context of post-genome sciences. The SG project started in April 2003 and will continue for 3 years in the framework of NEDO ( New Energy and Industrial Technology Organization) under the METI ( the Ministry of Economy, Trade, and Industry), Japan. The main purpose of the project is the development of high-throughput and robust machines, which should greatly contribute to the structural analysis of complex glycans. In this chapter, 2 major research items, i.e., ( 1) glycoproteomics, which enables comprehensive analysis of glycoproteins, and ( 2) "glycan profiling" by means of lectins, are described. For the latter, frontal affinity chromatography has been adopted as a starting tool for comprehensive analysis of the interaction of 100 lectins and 100 oligosaccharides under the concept of "hect-by-hect," which refers to 100 x 100.