Domain 36 of tropoelastin in elastic fiber formation.
Domain 36 of tropoelastin in elastic fiber formation.
复制标题
弹性纤维形成中弹性蛋白原的结构域 36。
DOI：
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发表时间：
2014
影响因子：
2
通讯作者：
H. Wachi
中科院分区：
文献类型：
--
作者：
Risa Nonaka;Fumiaki Sato;H. Wachi
弹性纤维组装是一个复杂的逐步过程，涉及多种不同的蛋白质和酶。由弹性蛋白基因的最后一个外显子编码的结构域36被认为是沉积到微纤维上的重要结构域，微纤维是弹性纤维组装的重要步骤。然而，域36在弹性纤维组装中的作用尚未阐明。在这里，我们利用我们建立的体外组装模型，以确定域36的组装过程的重要性。我们的研究结果表明，缺乏域36在牛原弹性蛋白的结果在缺陷的弹性纤维组装。用两个半胱氨酸残基的点突变和结构域36中的赖氨酸-精氨酸-赖氨酸-精氨酸（RKRK）序列的缺失获得了类似的结果。双免疫荧光的原弹性蛋白和Esclatin-1，微纤维的主要成分，证明这些突变的原弹性蛋白分子的Esclatin-1纤维上的本地化减少。此外，这些突变体的结合亲和力，以tumorin-1和微纤维相关糖蛋白（MAGP）显着降低。这些数据表明，弹性蛋白原的结构域36通过经由两个半胱氨酸残基和RKRK序列与微原纤维相互作用来促进弹性纤维组装。
Elastic fiber assembly is a complex stepwise process involving multiple different proteins and enzymes. Domain 36, encoded by the last exon of the elastin gene, is recognized to be an important domain for deposition onto microfibrils, an essential step in elastic fiber assembly. However, the role of domain 36 in elastic fiber assembly has not been clarified. Here, we utilized our established in vitro assembly model to identify the importance of domain 36 for the assembly process. Our results showed that the lack of domain 36 in bovine tropoelastin results in deficient elastic fiber assembly. A similar result was obtained with the point mutation of two cysteine residues and the deletion of the Lysine-Arginine-Lysine-Arginine (RKRK) sequence in domain 36. Double immunofluorescence of tropoelastin and fibrillin-1, a main component of microfibrils, demonstrated reduced localization of these mutant tropoelastin molecules on fibrillin-1 fibers. Moreover, the binding affinity of these mutants to fibrillin-1 and microfibril-associated glycoprotein (MAGP) was significantly decreased. These data indicate that domain 36 of tropoelastin facilitates elastic fiber assembly by interacting with microfibrils via two cysteine residues and the RKRK sequence.
DOI：
10.1042/bj20061145
发表时间：
2007-02
期刊：
The Biochemical journal
影响因子：
--
作者：
H. Wachi;Fumiaki Sato;Junji Nakazawa;Risa Nonaka;Z. Szabó;Z. Urban;T. Yasunaga;I. Maeda;K. Okamoto;B. Starcher;Dean Y Li;R. Mecham;Y. Seyama
通讯作者：
H. Wachi;Fumiaki Sato;Junji Nakazawa;Risa Nonaka;Z. Szabó;Z. Urban;T. Yasunaga;I. Maeda;K. Okamoto;B. Starcher;Dean Y Li;R. Mecham;Y. Seyama
原弹性蛋白的羧基末端在其组装成弹性纤维中的作用。
影响因子：
2.9
作者：
Hsiao,H;Stone,PJ;Toselli,P;Rosenbloom,J;Franzblau,C;Schreiber,BM
通讯作者：
Schreiber,BM