Regulation of expression of megakaryocyte and platelet proteoglycans.
Regulation of expression of megakaryocyte and platelet proteoglycans.
复制标题
巨核细胞和血小板蛋白多糖表达的调节。
DOI：
10.1002/stem.5530140729
复制
发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Schick,BP
中科院分区：
文献类型：
--
作者：
Schick,BP
蛋白多糖在造血细胞中的存在已被认识多年。然而，这些分子的结构和功能的阐明只是在最近几年才开始探索。本文综述了巨核细胞和巨核细胞肿瘤细胞中丝甘素蛋白聚糖的结构、功能和代谢的研究现状。我们已经确定了丝甘肽蛋白聚糖在调节总体流体动力学大小、糖胺聚糖链长度和二糖组成方面的复杂代谢模式，以及在对照细胞中或在佛波醇12-肉豆蔻酸酯13-乙酸酯或二甲亚砜存在下核心蛋白的加工。我们目前正在研究通过分析巨核细胞和非巨核细胞造血细胞中的启动子结构来调节这种蛋白质的合成。我们还初步鉴定了第二种蛋白聚糖β聚糖，也称为III型转化生长因子β受体。我们通过存在92 - 120 kDa之间的特征性核心蛋白，通过其粘附于辛基琼脂糖凝胶的能力和通过检测mRNA，在人红白血病和CHRF 288 - 11细胞中鉴定了该分子。我们希望将这些细胞中蛋白多糖代谢的研究应用于了解巨核细胞中α颗粒和膜元件的发育。
The existence of proteoglycans in hematopoietic cells has been recognized for many years. However, elucidation of the structure and function of these molecules has only begun to be explored in recent years. This paper reviews the current status of knowledge of the structure, function and metabolism of the serglycin proteoglycan in megakaryocytes and megakaryocyte tumor cells. We have identified complex metabolic patterns of the serglycin proteoglycan in terms of regulation of overall hydrodynamic size, glycosaminoglycan chain length and disaccharide composition, and processing of the core protein in control cells or in the presence of phorbol 12‐myristate 13‐acetate or dimethylsulfoxide. We are currently studying the regulation of synthesis of this protein by analysis of promoter constructs in megakaryocytic and non‐megakaryocytic hematopoietic cells. We have also tentatively identified a second proteoglycan, betaglycan, which is known also as the Type III transforming growth factor β receptor. We have identified this molecule in human erythroleukemia and CHRF 288‐11 cells by the presence of characteristic core proteins between 92‐120 kDa, by its ability to adhere to Octyl Sepharose and by detection of mRNA. We hope to apply studies of proteoglycan metabolism in these cells to understanding the development of alpha granules and membrane elements in megakaryocytes.