Species diversity and phyleogeography of Cornus kousa (Asian dogwood) captured by genomic and genic microsatellites
Species diversity and phyleogeography of Cornus kousa (Asian dogwood) captured by genomic and genic microsatellites
复制标题
基因组和基因微卫星捕获的山茱萸（亚洲山茱萸）的物种多样性和系统地理学
DOI：
10.1002/ece3.6537
复制
发表时间：
2020
影响因子：
4.1
通讯作者：
Robert N. Trigiano
中科院分区：
文献类型：
--
作者：
Marcin Nowicki;Logan C. Houston;Sarah L. Boggess;Anthony S. Aiello;Miriam Pay Milans;Margaret E. Staton;Mitsuhiro Hayashida;Masahiro Yamanaka;Shigetoshi Eda;Robert N. Trigiano
亚洲山茱萸（Cornus kousa）是一种东亚本土树木，是山茱萸属中最重要的经济物种，由于其理想的园艺特性和与北美本土山茱萸杂交的能力。为了评估物种的遗传多样性，并更好地为正在进行的和未来的育种工作提供信息，我们收集了131个非栽培的C. kousaspecimens的植物标本馆和植物园。利用新开发的基因微卫星和已发表的核基因组微卫星对我们的库萨采集物进行基因分型和毛细管电泳分析，可以评估该物种的遗传多样性和进化历史。无论使用的微卫星类型如何，该研究对C. kousa多样性产生了大致相似的见解，其中细微的差异来自并强调了所使用的标记。积累的证据表明，物种的独特基因库与植物原产国有关。这将有助于这一重要观赏作物的商业品种的开发，增加了植物的实用性状。使用累积的基因分型数据集对库萨进化历史进行的分析指出，根据该地区的地理位置，一个未采样的祖先种群可能已经灭绝。据我们所知，很少有研究利用相同的gDNA集合来比较基因组和基因微卫星的性能。这是首次详细报道龟背竹物种多样性和进化历史推断。
Cornus kousa(Asian dogwood), an East Asia native tree, is the most economically important species of the dogwood genus, owing to its desirable horticultural traits and ability to hybridize with North America‐native dogwoods. To assess the species genetic diversity and to better inform the ongoing and future breeding efforts, we assembled an herbarium and arboretum collection of 131 noncultivatedC. kousaspecimens. Genotyping and capillary electrophoresis analyses of ourC. kousacollection with the newly developed genic and published nuclear genomic microsatellites permitted assessment of genetic diversity and evolutionary history of the species. Regardless of the microsatellite type used, the study yielded generally similar insights into theC. kousadiversity with subtle differences deriving from and underlining the marker used. The accrued evidence pointed to the species distinct genetic pools related to the plant country of origin. This can be helpful in the development of the commercial cultivars for this important ornamental crop with increased pyramided utility traits. Analyses of theC. kousaevolutionary history using the accrued genotyping datasets pointed to an unsampled ancestor population, possibly now extinct, as per the phylogeography of the region. To our knowledge, there are few studies utilizing the same gDNA collection to compare performance of genomic and genic microsatellites. This is the first detailed report onC. kousaspecies diversity and evolutionary history inference.