A Novel Method for Efficient Collection of Normal Mesothelial Cells In Vivo
A Novel Method for Efficient Collection of Normal Mesothelial Cells In Vivo
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DOI：
10.3164/jcbn.10-01
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发表时间：
2010-05-01
影响因子：
2.4
通讯作者：
Toyokuni, Shinya
中科院分区：
文献类型：
--
作者：
Jiang, Li;Yamashita, Yoriko;Toyokuni, Shinya
石棉诱发的间皮瘤在许多国家是一个具有挑战性的社会问题，而通过铁引起的氧化应激与其致癌密切相关。间皮瘤被认为起源于覆盖体腔的间皮细胞，如胸膜、心包和腹膜腔。它们是单层的，非常扁平，很难从实验动物身上获得纯间皮细胞作为对照样本。在这里，我们描述了一种新的方法，通过使用简单的设备从动物身上收集间皮细胞。肺、脾、肝等脏器表面覆盖单层间皮细胞。在液氮中快速冷冻后，用剃须刀片刮这些器官的表面，可以令人满意地获得几乎纯净的间皮细胞。这种简单的方法将有助于从动物身上获得间皮对照标本，以阐明各种间皮病理的分子机制。
Asbestos-induced mesothelioma is a challenging social problem in many countries, and oxidative stress via iron is closely associated with its carcinogenesis. Mesothelioma is thought to originate from the mesothelial cells that cover the somatic cavity such as pleural, pericardial and peritoneal cavities. They are single layered and so flat that it is extremely difficult to obtain pure mesothelial cells as control samples from experimental animals. Here we describe a novel method to collect mesothelial cells from animals by the use of simple equipments. Surface of the most organs including lung, spleen and liver are covered with a single layer of mesothelial cells. Scraping the surface of those organs with razor blades after snap-freeze in liquid nitrogen satisfactorily confers almost pure population of mesothelial cells. This simple method would be helpful for obtaining mesothelial control samples from animals to elucidate the molecular mechanisms of a variety of mesothelial pathology.