Ras1 promotes cellular growth in the Drosophila wing
Ras1 promotes cellular growth in the Drosophila wing
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DOI：
10.1016/s0092-8674(00)80679-0
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发表时间：
2000-02-18
期刊：
影响因子：
64.5
通讯作者：
Edgar, BA
中科院分区：
文献类型：
--
作者：
Prober, DA;Edgar, BA
Ras GTP酶将细胞外有丝分裂原与控制细胞增殖的细胞内机制联系起来。为了了解Ras如何调节体内增殖，我们在发育中的果蝇翅膀的细胞克隆中激活或灭活Ras。缺乏Ras的细胞较小，生长速率降低，在G1期积累，并由于细胞竞争而发生凋亡。相反，Ras的激活增加了细胞的大小和生长速率，并促进了G1/S转换。Ras上调生长驱动因子dMyc，Pas和dMyc均在转录后增加细胞周期蛋白E的水平。我们认为Pas主要促进生长，并且生长通过细胞周期蛋白E与G1/S进展偶联。有趣的是，Pas对生长的上调并没有解除G2/M期进展或发育调节的细胞周期退出。
The Ras GTPase links extracellular mitogens to intracellular mechanisms that control cell proliferation. To understand how Ras regulates proliferation in vivo, we activated or inactivated Ras in cell clones in the developing Drosophila wing. Cells lacking Ras were smaller, had reduced growth rates, accumulated in G1, and underwent apoptosis due to cell competition. Conversely, activation of Ras increased cell size and growth rates and promoted G1/S transitions. Ras upregulated the growth driver dMyc, and both Pas and dMyc increased levels of cyclin E posttranscriptionally. We propose that Pas primarily promotes growth and that growth is coupled to G1/S progression via cyclin E. Interestingly, upregulation of growth by Pas did not deregulate G2/M progression or a developmentally regulated cell cycle exit.