Biochemical characterization of serum thyroglobulin from patients with bone metastases from follicular carcinoma of the thyroid.
Biochemical characterization of serum thyroglobulin from patients with bone metastases from follicular carcinoma of the thyroid.
复制标题
甲状腺滤泡癌骨转移患者血清甲状腺球蛋白的生化特征。
DOI：
--
复制
发表时间：
1991
影响因子：
1.4
通讯作者：
U. Thakare
中科院分区：
文献类型：
--
作者：
A. Kumar;D. H. Shah;U. Thakare
本文研究了6例甲状腺滤泡癌及远处骨转移患者血清甲状腺球蛋白的生化特性。由于很难从血清样品中分离出足够的甲状腺球蛋白，因此使用放射性碘给药后获得的体内放射性血清甲状腺球蛋白。与从正常甲状腺组织中分离的天然甲状腺球蛋白观察到的尖锐盐析模式相反，转移血清甲状腺球蛋白观察到广泛的盐析曲线。离子交换柱低离子强度洗脱转移性血清甲状腺球蛋白。95%以上的转移性血清甲状腺球蛋白能与刀豆蛋白- a sepharose结合，并用0.5 M α -甲基甘露糖洗脱。转移性血清甲状腺球蛋白和天然甲状腺球蛋白对刀豆蛋白a的反应性相当。两种类型的甲状腺球蛋白在蔗糖线性密度梯度离心下表现出相同的流动性。因此，甲状腺滤泡癌的转移性血清甲状腺球蛋白表现为19s甲状腺球蛋白，其结合糖接近正常，但表面电荷改变。
The biochemical properties of serum thyroglobulin obtained from six patients with follicular carcinoma of the thyroid and distant metastases to bone(s) have been studied. Since it is difficult to isolate sufficient thyroglobulin from serum samples, in vivo radioiodinated serum thyroglobulin obtained after radioiodine administration was used. In contrast to a sharp salting-out pattern observed with native thyroglobulin isolated from normal thyroid tissue, a broad salting-out curve was seen with metastatic serum thyroglobulin. The metastatic serum thyroglobulin eluted with low ionic strength from ion-exchange column. More than 95% of metastatic serum thyroglobulin could be bound to concanavalin-A sepharose and be eluted with 0.5 M alpha-methyl mannoside. The reactivity of metastatic serum thyroglobulin and native thyroglobulin towards concanavalin-A was comparable. Both types of thyroglobulins showed identical mobilities on sucrose linear density gradient centrifugation. The metastatic serum thyroglobulin from follicular carcinoma of the thyroid thus appears to be 19 S thyroglobulin with near normal concanavalin-A binding sugars but altered surface charges.