Influence of biofilms on microbial contamination in dental unit water.
Influence of biofilms on microbial contamination in dental unit water.
复制标题
生物膜对牙科综合治疗台水中微生物污染的影响。
DOI：
10.1016/0300-5712(91)90075-a
复制
发表时间：
1991
影响因子：
4.4
通讯作者：
C. Lilge
中科院分区：
文献类型：
--
作者：
R. Whitehouse;E. Peters;J. Lizotte;C. Lilge
牙科综合治疗台 (DU) 中用于冷却和清洁牙科手术区域的水经常受到微生物污染。口腔微生物逆行扩散到 DU 管中、受污染的管道系统和内源性 DU 污染都与此有关。本研究调查了 11 个随机选择的 DU 中 DU 管对这种污染的影响。在标准化条件下，测量了 DU 细菌水平因 DU 操作引起的冲洗而降低或因关闭 DU 引起的停滞而增加所需的时间。然后移除 DU 管并进行类似操作。结果显示 DU 及其相应管中的细菌水平和种群相似。经过一段时间的停滞导致 DU 细菌污染后，从远处的连接管道系统中采集的 16 个对照样本呈阴性。这表明管道在我们的系统中并不是一个重要因素。因此，DU 可以内源性地污染流经它们的水；它们的试管有可能产生与完整 DU 所确定的类似程度的细菌污染。管腔的扫描电子显微镜显示了生物膜，其特征是在所有情况下微生物都嵌入无定形基质中。这种生物膜可以充当水库，促进快速再污染。对数据的进一步分析表明可能还有其他影响因素。
Water from dental units (DU), used for cooling and clearing the field of dental operations, is frequently contaminated by microorganisms. Retrograde spread of oral microbes into DU tubing, contaminated plumbing systems and endogenous DU contamination have been implicated This study investigated the contribution of DU tubing to this contamination in 11 randomly selected DU. The times required, under standardized conditions, for DU bacterial levels to decrease in response to the flushing caused by DU operation, or increase in response to stagnation caused by shutting down the DU, were measured. The DU tubing was then removed and similarly manipulated. The results showed similar bacterial levels and populations in the DU and their corresponding tubes. Sixteen control samples taken from the connecting plumbing system at distant locations, after periods of stagnation which result in DU bacterial contamination, were negative. This suggests the plumbing, in our system, is not an important factor. Thus, DU can endogenously contaminate the water passing through them; their tubes have the potential to generate similar magnitudes of bacterial contamination to that determined from intact DU. Scanning electron microscopy of the tube lumens showed a biofilm, characterized by microorganisms embedded in an amorphous matrix in all cases. This biofilm could act as a reservoir to facilitate rapid recontamination. Further analysis of the data indicates there could be other contributing factors.